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Related Experiment Video

Updated: Sep 11, 2025

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
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Myosin cluster dynamics determines epithelial wound ring constriction.

Alka Bhat1,2,3,4,5, Rémi Berthoz1,2,3,4,5, Simon Lo Vecchio1,2,3,4,5

  • 1Laboratory of Cell Physics ISIS/IGBMC, CNRS and University of Strasbourg, Strasbourg, France.

Iscience
|August 13, 2025
PubMed
Summary
This summary is machine-generated.

Myosin and actin clusters self-assemble in cells and tissues. Their conserved dynamics in single and multicellular systems offer insights into morphogenesis and wound healing.

Keywords:
Cell biologyOrganizational aspects of cell biology

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Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers
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Area of Science:

  • Cell Biology
  • Biophysics
  • Developmental Biology

Background:

  • Myosin-actin clusters self-assemble into submicrometric structures regulated by RhoA.
  • These clusters exhibit emergent dynamics in various morphogenetic systems, including Drosophila and in vitro actomyosin assays.
  • In single-cell systems, cluster dynamics influence stress generation (radial) and transport (tangential) during cytokinesis.

Purpose of the Study:

  • To investigate the dynamics of actomyosin clusters in multicellular rings during epithelial wound closure.
  • To correlate cluster dynamics with RhoA activity in multicellular systems.
  • To determine if actomyosin cluster dynamics are conserved across single and multicellular systems.

Main Methods:

  • Utilized FRET sensors to assess RhoA activity in epithelial monolayers undergoing wound closure.
  • Observed and analyzed the dynamics of actomyosin clusters within multicellular rings.
  • Compared cluster dynamics in multicellular systems to known dynamics in single-cell systems.

Main Results:

  • Actomyosin cluster dynamics in multicellular rings during wound closure mirror those in single-cell systems.
  • Cluster dynamics were found to be uncorrelated with RhoA activity.
  • Bursts of RhoA activation were observed to precede myosin recruitment.

Conclusions:

  • Actomyosin cluster dynamics are conserved between single-cell and multicellular systems.
  • These conserved dynamics suggest myosin clusters can serve as generic indicators for stress generation and shape changes in morphogenesis.
  • Understanding these dynamics can aid in predicting and mapping morphogenetic processes like wound healing.