Comparing reliability-based measures of functional connectivity between movie and rest: An ROI-based approach

Area of Science:

• Neuroscience
• Psychiatry
• Brain Imaging

Background:

• Functional connectivity (FC) is crucial for precision psychiatry but needs improved reliability and sensitivity.
• Movie watching offers potential advantages for whole-brain FC measures in clinical applications.
• The utility of movie-watching FC in specific, psychiatrically relevant brain regions remains under investigation.

Purpose of the Study:

• To compare the reliability and discriminability of movie-watching versus resting-state FC.
• To assess FC measures in key brain regions for precision psychiatry.
• To evaluate the performance of movie-watching FC across the entire brain.

Main Methods:

• Compared univariate and multivariate reliability measures for movie-watching and resting-state FC.
• Analyzed FC data in dorsolateral prefrontal cortex, presupplementary motor area, and temporoparietal junction.
• Expanded analysis to all brain regions using image intraclass correlation coefficients (I2C2), discriminability, and fingerprinting.

Main Results:

• Movie-watching FC reliability was comparable to resting-state FC in specific regions (dorsolateral prefrontal cortex, presupplementary motor area).
• Movie-watching FC demonstrated higher discriminability in the temporoparietal junction.
• Across all regions, movie watching improved discriminability in 25% of parcels and 'fingerprinting' accuracy in 59 parcels (visual/temporal regions).

Conclusions:

• Movie watching is a viable alternative to resting-state for FC acquisition in research settings.
• This approach may optimize FC discriminability for individualized brain measures in psychiatry.
• An interactive tool is provided for researchers to explore cross-state FC reliability differences.