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CD45 and CD148 Are Critically Involved in Neutrophil Recruitment and Function During Inflammatory Arthritis in Mice
Jan-Niklas Heming1, Andreas Margraf1,2, Karolina Najder1
1Department of Anesthesiology, Intensive Care and Pain Medicine, University Hospital Münster, 48149 Münster, Germany.
Cells
|August 13, 2025
Summary
CD45 and CD148 phosphatases regulate neutrophil function in inflammatory arthritis. CD45 promotes neutrophil infiltration and activation, while CD148 has dual roles, impacting arthritis onset and reactive oxygen species production.
Area of Science:
- Immunology
- Cell Biology
Background:
- Neutrophils are critical immune cells involved in autoimmune diseases like rheumatoid arthritis.
- Receptor-like tyrosine phosphatases (RPTPs) CD45 and CD148 are implicated in immune cell regulation.
Purpose of the Study:
- To investigate the distinct roles of CD45 and CD148 in regulating neutrophil function during inflammatory arthritis.
Main Methods:
- Utilized an in vivo K/BxN serum transfer-induced arthritis mouse model.
- Analyzed neutrophil infiltration, cytokine release, reactive oxygen species (ROS) production, and Src family kinase activation.
Main Results:
- CD45 deficiency impaired neutrophil infiltration, cytokine release, and ROS production.
- CD148 deficiency delayed arthritis onset, reduced ROS production, but did not alter overall neutrophil infiltration.
- CD45 and CD148 differentially regulated Src family kinase activation in neutrophils.
Conclusions:
- CD45 positively regulates neutrophil recruitment and function in inflammatory arthritis.
- CD148 exhibits both positive and negative regulatory roles in neutrophil recruitment and function.
- Distinct roles of CD45 and CD148 offer potential therapeutic targets for inflammatory arthritis.
