IL-2 and IFN-γ Secretion of Activated Jurkat T Cells via a Microdroplet-SERS based Single-Cell Immunoassay...

Studying the characteristics of T cell activation and cytokine secretion is crucial for understanding the cell-mediated immunological response (CMI). To assess this purpose, we present a droplet-based single-cell immunoassay platform, named Drop-SCIA, which uniquely integrates surface-enhanced Raman spectroscopy (SERS) with homogeneous-phase immunoassay, enabling highly sensitive, multiplexed cytokine detection at the single-cell level and offering superior target enrichment efficiency compared to the widely used interface-based ELISpot assay. Using this platform, we analyzed Jurkat T cell activation and further profiled IL-2 and IFN-γ secretion following coculture with normal breast epithelial cells (MCF-10A) and breast cancer subtypes (MCF-7, MDA-MB-231). The platform enables robust and reliable single-cell immune profiling of Jurkat cells, allowing precise assessment of their activation status. Significant differences in IL-2 and IFN-γ secretion were observed under different coculture conditions, indicating that distinct breast cancer subtypes exert differential effects on T cell activation and function. By combining microdroplet microfluidics with SERS-based signal readout, Drop-SCIA represents a significant technological advancement in the study of CMI within tumor microenvironments. It will aid in the precise analysis of T-cell immune function for vaccine development, cancer immunotherapy, and the diagnosis and treatment of viral and other infectious diseases.