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Extracellular ssDNA from <i>Pittosporum tobira</i> Exerts Strong Insecticidal Activity on <i>Coccus hesperidum</i>: A Natural Parallel to 'Genetic Zipper' Technology.

International journal of molecular sciences·2026
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Comparative Phenotype and Transcriptome Profiling in Some Grapevine Cultivars in Response to Drought Stress.

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Contact Unmodified Antisense DNA Biotechnology (CUADb)-Based Oligonucleotide Insecticides and RNA Biocontrols: Molecular Bases and Potential in Plant Protection.

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Ribosomal RNA-Specific Antisense DNA and Double-Stranded DNA Trigger rRNA Biogenesis and Insecticidal Effects on the

Vol Oberemok1,2, Nikita Gal'chinsky1, Ilya Novikov1

  • 1Department of General Biology and Genetics, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Russia.

International Journal of Molecular Sciences
|August 14, 2025
PubMed
Summary

Short antisense DNA oligonucleotides offer a novel, eco-friendly approach to insect pest control by targeting rRNA. This DNA-based strategy effectively reduces pest populations, presenting a new frontier in crop protection.

Keywords:
DNA containment mechanism (DNAc)DNA-programmable plant protectioncontact unmodified antisense DNA biotechnology (CUADb)genetic zipper methodkinase disasteroligonucleotide insecticides

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Area of Science:

  • Biotechnology
  • Entomology
  • Molecular Biology

Background:

  • Contact unmodified antisense DNA biotechnology (CUADb) uses DNA oligonucleotides to control insect pests.
  • Sap-feeding insects are economically damaging and vectors of plant viruses.
  • CUADb targets pest mature and pre-ribosomal RNA (rRNA).

Purpose of the Study:

  • To evaluate the insecticidal efficacy of short 11-mer antisense DNA oligos against Coccus hesperidum.
  • To compare short oligos with long single-stranded and double-stranded DNA sequences.
  • To elucidate the DNA containment (DNAc) mechanism and its molecular interactions with rRNA.

Main Methods:

  • Application of short 11-mer and long 56-mer antisense DNA oligonucleotides.
  • Insect mortality assessment over 9 days.
  • Analysis of rRNA levels, ribosome biogenesis, ATP production, and enzyme activity (RNase H1, RNAi enzymes).

Main Results:

  • Short 11-mer oligos showed higher insecticidal activity (97.66% mortality) than long sequences (77.09%).
  • DNAc induced metabolic shifts, leading to a 'kinase disaster' via rRNA degradation and kinase downregulation.
  • Perfect rRNA complementarity was crucial for significant rRNA degradation and insect mortality, mediated by RNase H1, with distinct mechanisms from RNAi.

Conclusions:

  • Short antisense DNA oligos are highly effective insecticides against Coccus hesperidum.
  • The DNA containment mechanism involves rRNA degradation triggered by perfect sequence complementarity.
  • CUADb represents an innovative, eco-friendly DNA-programmable pest control strategy with potential for crop protection.