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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

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The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Insights of Surface Enhancing Raman Spectroscopy for Biomedical Application.

Neha Sharma1, Anita Singh1, Ratneshwar Kumar Ratnesh2

  • 1Department of Pharmaceutical Technology, Meerut Institute of Engineering & Technology Meerut, Uttar Pradesh 250005, India.

Methods (San Diego, Calif.)
|August 14, 2025
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Summary

Surface-Enhanced Raman Spectroscopy (SERS) offers rapid, sensitive, and specific molecular detection for biomedical diagnostics. This review highlights SERS applications in diagnosing diseases like cancer and COVID-19, and its future potential.

Keywords:
Biological SciencesBiomedicalDiagnosisNanostructureRaman scatteringSurface Chemistry

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Spectroscopy

Background:

  • Raman spectroscopy provides non-invasive molecular identification via spectral fingerprints.
  • Surface-Enhanced Raman Spectroscopy (SERS) amplifies signals using metal nanoparticles for enhanced sensitivity.
  • Conventional diagnostics can be slow, posing challenges in critical care settings.

Purpose of the Study:

  • To review the integration of Raman spectroscopy, particularly SERS, for in vivo and ex vivo biomedical diagnostics.
  • To explore sample preparation, spectral interpretation, and biomarker correlation in SERS-based diagnostics.
  • To focus on SERS applications in diagnosing neurological disorders, cancers, drug abuse, and COVID-19.

Main Methods:

  • Review of existing literature on Raman spectroscopy and SERS in biomedical applications.
  • Analysis of SERS techniques for sample preparation and spectral data interpretation.
  • Examination of SERS correlation with disease biomarkers for diagnostic purposes.

Main Results:

  • SERS offers a sensitive, specific, and rapid alternative to conventional bioanalytical methods.
  • Raman-based methods show promise in diagnosing a range of conditions including brain disorders, cancers, and infectious diseases.
  • The technique requires minimal sample preparation, facilitating faster results.

Conclusions:

  • Raman spectroscopy, especially SERS, is a powerful tool for advanced biomedical diagnostics.
  • Continued development of SERS technologies is crucial for improving clinical outcomes.
  • Future research should address challenges to further advance SERS in healthcare.