Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

7.1K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
7.1K
Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

6.5K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
6.5K
Sign Test for Matched Pairs01:17

Sign Test for Matched Pairs

208
The sign test for matched pairs offers a robust method for comparing two paired samples, often for the effects of an intervention in one of them. This method is very useful in situations where the underlying distribution of the data is unknown. The test compares two related samples—often pre- and post-treatment measurements on the same subjects—to determine if there are significant differences in their median values.
To conduct the sign test, we first calculate the differences in...
208
Color Vision01:24

Color Vision

698
Color perception begins in the retina, the light-sensitive layer at the back of the eye. Two main theories explain how colors are seen: the trichromatic theory and the opponent-process theory. The trichromatic theory, proposed by Thomas Young in 1802 and extended by Hermann von Helmholtz in 1852, suggests that color vision is based on three types of cone receptors in the retina. These cones are sensitive to different but overlapping ranges of wavelengths corresponding to red, blue, and green.
698

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Meta-analysis of defocus curves of monofocal, enhanced monofocal and extended depth of focus IOLs.

BMJ open ophthalmology·2025
Same author

A review of angle kappa and multifocal intraocular lenses and their effect on visual outcomes.

Acta ophthalmologica·2025
Same author

Ethnicity-Based Differences in Corneal Asphericity: Intraocular Lens Power Calculation, Image Quality, and Visual Acuity After Cataract Surgery Using Eye Models.

Clinical ophthalmology (Auckland, N.Z.)·2025
Same author

[Alcohol Dependence: one or more nosological entities? Identifying geographical diversities among patients through DSM-4 and Artificial Intelligence].

Epidemiologia e prevenzione·2025
Same author

Impact of Aspheric Monofocal Intraocular Lens Implantation on Uncorrected Intermediate Visual Acuity: a Combined Analysis.

Clinical ophthalmology (Auckland, N.Z.)·2024
Same author

Population mortality before and during the COVID-19 epidemic in two Sudanese settings: a key informant study.

BMC public health·2024

Related Experiment Video

Updated: Sep 11, 2025

Training Synesthetic Letter-color Associations by Reading in Color
10:27

Training Synesthetic Letter-color Associations by Reading in Color

Published on: February 20, 2014

23.0K

Letter contrast sensitivity validation.

Michel Guillon1, Pasquale Pepe1, Jessie Hull2

  • 1Ocular Technology Group - International, London, UK.

Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)
|August 16, 2025
PubMed
Summary
This summary is machine-generated.

The computerized letter contrast sensitivity (CS) test demonstrated high repeatability and lower test variance compared to sinusoidal methods. This validated its use for assessing functional vision across various conditions.

Keywords:
computerised letter testcontrast sensitivitysinusoidal testtest–retest repeatability

More Related Videos

Development of a Gaze-Contingent Display Framework Designed for Perceptual and Oculomotor Research with Simulated Central Vision Loss
07:12

Development of a Gaze-Contingent Display Framework Designed for Perceptual and Oculomotor Research with Simulated Central Vision Loss

Published on: April 11, 2025

560
Measuring the Behavioral Effects of Intraocular Scatter
05:10

Measuring the Behavioral Effects of Intraocular Scatter

Published on: February 18, 2021

3.5K

Related Experiment Videos

Last Updated: Sep 11, 2025

Training Synesthetic Letter-color Associations by Reading in Color
10:27

Training Synesthetic Letter-color Associations by Reading in Color

Published on: February 20, 2014

23.0K
Development of a Gaze-Contingent Display Framework Designed for Perceptual and Oculomotor Research with Simulated Central Vision Loss
07:12

Development of a Gaze-Contingent Display Framework Designed for Perceptual and Oculomotor Research with Simulated Central Vision Loss

Published on: April 11, 2025

560
Measuring the Behavioral Effects of Intraocular Scatter
05:10

Measuring the Behavioral Effects of Intraocular Scatter

Published on: February 18, 2021

3.5K

Area of Science:

  • Ophthalmology
  • Vision Science
  • Clinical Measurement

Background:

  • Contrast sensitivity (CS) is crucial for evaluating functional vision.
  • Assessing the full CS function requires evaluating multiple spatial frequencies.
  • Computerized tests offer a method to assess CS across various spatial frequencies.

Purpose of the Study:

  • To validate computerized letter and sinusoidal contrast sensitivity (CS) methods.
  • To determine the repeatability of CS measurements using different spatial frequencies and lighting conditions.

Main Methods:

  • A prospective study compared repeatability of CS measurements using three methods: computerized letter CS, computerized sinusoidal CS (M&S Technologies), and printed sinusoidal CS (VectorVision).
  • Measurements were taken under photopic and mesopic conditions (with/without glare).
  • Repeatability was assessed in 20 participants by comparing mean differences and 95% confidence intervals between visits.

Main Results:

  • Computerized letter CS showed significantly lower test variance than M&S or VectorVision instruments across most spatial frequencies.
  • Letter CS exhibited a lower precision-to-tolerance ratio and required a smaller sample size for comparable precision.
  • The computerized letter method demonstrated superior repeatability.

Conclusions:

  • The computerized letter method for assessing CS function is highly repeatable.
  • This method offers significantly lower test variance compared to sinusoidal instruments.
  • Computerized letter CS is a reliable tool for evaluating functional vision.