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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Breaking Acid-Induced Fluorescence Quenching in Fluorescein Derivatives: Surfactant-Directed Aggregation Control for

Xueshan Chen1, Yao Lin2, Yuyang Li2

  • 1Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China.

Small (Weinheim an Der Bergstrasse, Germany)
|August 18, 2025
PubMed
Summary

Fluorescein derivatives lose optical properties in acid, but surfactants restore fluorescence. This enables a novel, fast, and low-cost anti-counterfeiting system using inkjet-printed dyes and surfactant solutions.

Keywords:
aggregationencryption & anti‐counterfeitingfluorescein derivativesmulti‐channelsurfactant‐activated

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Area of Science:

  • Chemical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Fluorescein derivatives suffer from optical property loss due to proton attack and aggregation-caused quenching.
  • Spirocyclization and aggregation-related quenching limit the applications of fluorescein derivatives.

Purpose of the Study:

  • To overcome the limitations of fluorescein derivatives in acidic conditions by restoring their optical properties.
  • To develop a novel encryption strategy based on surfactant-activated optical performance of fluorescein derivatives.

Main Methods:

  • Experimental characterizations and theoretical calculations were used to elucidate the mechanism of fluorescence recovery.
  • Surfactant-assembled dispersions were employed to prevent quenching.
  • Inkjet printing technology was utilized for dye loading onto fiber paper.

Main Results:

  • Surfactants restored and enhanced optical properties by proton sequestration and colloidal redispersion, achieving a ≈2150-fold fluorescence increase at pH 1.0.
  • A novel encryption strategy was developed utilizing proton-induced molecular aggregation for chromogenic quenching and fluorescence suppression.
  • The developed system demonstrated fast response (1 s), low cost, and multi-channel encryption capabilities.

Conclusions:

  • Surfactant-mediated strategies can effectively overcome the limitations of fluorescein derivatives, enabling programmable fluorescence recovery.
  • The developed encryption system offers a user-friendly, multi-channel solution with broad applications in anti-counterfeiting and information security.
  • This approach paves the way for advanced intelligent packaging and anti-counterfeiting technologies.