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Updated: Sep 11, 2025
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Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles
Published on: February 7, 2025
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Myoblast and fibroblast derived small extracellular vesicles differentially affect myoblast migration dynamics
Kyle N Hagemann1, Rhys S McColl1, Jason A C Lovett1
1Department of Physiological Sciences, Stellenbosch University, Stellenbosch, 7600, South Africa.
Journal of Muscle Research and Cell Motility
|August 18, 2025
Summary
Muscle injury involves communication via extracellular vesicles (EVs). This study shows myoblast-derived EVs enhance myoblast migration, while fibroblast-derived EVs impair it, highlighting cell-specific EV roles in muscle regeneration.
Area of Science:
- Biochemistry
- Cell Biology
- Regenerative Medicine
Background:
- Muscle injury involves satellite cells and fibroblasts, with extracellular vesicles (EVs) mediating intercellular communication.
- The specific roles of EVs from different cell types in muscle regeneration remain unclear.
Purpose of the Study:
- To investigate the uptake and effects of EVs derived from C2C12 myoblasts (myo-EVs) and L929 fibroblasts (fibro-EVs) on proliferating myoblasts.
- To understand cell-specific EV-mediated communication in muscle regeneration.
Main Methods:
- Cultured C2C12 myoblasts and L929 fibroblasts in EV-depleted media.
- Isolated and characterized myo-EVs and fibro-EVs.
- Assessed EV uptake, myoblast proliferation, migration (scratch closure, leading front migration rate, individual cell trajectories), and MyoD/Myogenin expression.
Main Results:
- Myoblasts preferentially internalized myo-EVs over fibro-EVs at 5 and 48 hours.
- EVs did not affect myoblast proliferation but slightly increased Myogenin expression, suggesting a shift towards differentiation.
- Myo-EVs enhanced myoblast migration rate into a scratch.
- Fibro-EVs increased individual cell speed but impaired leading front migration due to reduced persistence.
Conclusions:
- Cell-specific EVs play distinct roles in regulating myoblast behavior.
- Myo-EVs promote migration, while fibro-EVs can hinder collective cell movement.
- Future applications using EVs for muscle regeneration should consider the source and purity of EVs to avoid confounding effects.
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