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Updated: Sep 11, 2025
Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
Published on: December 16, 2018
Characterization of human mesenchymal stem cell according to delivery route and its correlation with
Moises Martínez-Castillo1, Itzel Altamirano-Mendoza1,2, Benjamín León-Mancilla1,3
1Experimental Medicine Research Unit, Faculty of Medicine, Universidad Nacional Autónoma de México (UNAM).
Objective:
To assess the pluripotency profile of human mesenchymal stem cells (hMSCs) as influenced by delivery mode and obstetric-gynecological background.
Methods:
Thirty-nine placentas were included. A fragment of the AM (5 cm) was utilized to obtain the AM-hMSCs. The cell cultures were monitored until 90% confluence, before carrying out expansion (passages). The cultures, cell morphology, adhesion, immunophenotyping characterization, and differentiation capacity were evaluated and compared with hMSC from bone marrow (BM-hMSC). T-student and Mann-Whitney U tests were carried out. Statistical significance was set at a p < 0.05.
Results:
No differences were observed in the proliferation and expansion of AM-hMSC obtained by vaginal or cesarean delivery route. Similar results were observed in the immunological characterization; however, CD105 was significantly lower compared with BM-hMSC. Nevertheless, cells from vaginal or cesarean delivery route showed great differentiation capacity to adipogenic, chondrogenic, and osteogenic lineages.
Conclusions:
The delivery route, clinical data, and obstetric and gynecologic histories are no limitations for using the AM to obtain hMSC and its possible application in Regenerative Medicine.
