Single-Electrode Electrochemiluminescence System for High-Throughput Visual Analysis of Chloramphenicol

To achieve high-throughput visual detection, an innovative single-electrode multiarray electrochemiluminescence (SE-ECL) biosensing and mobile phone imaging platform is developed. For the first time, Au-luminol was applied to a single-electrode, eight-hole ECL array. The high-intensity luminophor of luminol combined with smartphone imaging technology supports synchronous ECL detection of multiple reaction units, increasing the detection flux to 8 times that of traditional methods while maintaining the signal consistency of each unit. The system is both portable and intelligent, providing the possibility for rapid on-site screening. Compared with the traditional three-electrode system, the single-electrode design drives the ECL reaction through the resistance-induced potential difference, which eliminates the complex electrode array, significantly reduces the manufacturing cost, simplifies the operation process, and greatly enhances the practicality. The work uses DNA cycle amplification technology to trigger the molecular gating mechanism of target response mesoporous silica nanoparticles (MSNs) to achieve specific recognition of CAP and double signal amplification, realizing sensitive mobile phone ECL imaging for CAP detection. This study not only provides a high-throughput, low-cost, and portable integrated technology for efficient monitoring of trace antibiotic pollution in the ocean but also creates a new paradigm of high-throughput sensing technology in the field of environmental analysis.