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An Optimized SPLiT-Seq Protocol for Insects

Julien Devilliers1,2, Elena Emili3,4, Virginia Vanni3,5

  • 1Neurogenetics Group, University of Leicester, Leicester, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2025
PubMed
Abstract

Keywords:
ACMECell dissociationInsectsSPLiT-seqSingle-cell transcriptomics

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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