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Improved transformation system of Bacillus subtilis competent cells using polymerase chain reaction DNA fragments as

Kazuhisa Sawada1,2, Keiji Endo1, Masatoshi Tohata1

  • 1Global R&D - Biological Science Research, Kao Corporation, Akabane 2606, Ichikai, Haga, Tochigi 3213497, Japan.

Bioscience, Biotechnology, and Biochemistry
|August 19, 2025
PubMed

Keywords:
Bacillus subtilisMarburg 168PCR DNAcompetent celltransformation

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Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
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