Storage of thawed cryoprecipitate for 5 days at refrigerated temperatures: A systematic review and meta-analysis

This systematic review and meta-analysis aim to evaluate the changes in coagulation factor and bacterial contamination by extending the preservation period of thawed cryoprecipitate for 5 days at refrigerated temperatures. Literature searches were conducted from three different databases, and the literature was screened according to the inclusion criteria. The quality of the literature was assessed using the Risk of Bias in Non-randomised Studies-of Interventions (ROBINS-I) tool, and a meta-analysis was conducted using RevMan5.3 software. Finally, five studies met the inclusion criteria. The meta-analysis results show that fibrinogen concentration remains stable after storing the thawed cryoprecipitate at refrigerated temperatures for 5 days Mean Difference (MD) = -0.0802, 95% Confidence Interval (CI) = -0.4220, 0.2616, p = 0.65. The systematic review showed that thawed cryoprecipitate stored at refrigerated temperatures for 120 h had a slight decrease in factor VIII activity but still met the current standard, von Willebrand factor content level remained relatively stable, factor XIII activity showed no significant decrease, and no bacterial contamination was detected. Overall, the available data suggest that fibrinogen concentration remained stable and no bacterial contamination was detected after storing the thawed cryoprecipitate at refrigerated temperatures for 5 days. The lack of assessment of aggregate formation is an important limitation of this review. In addition, the number of relevant studies is still relatively limited, and further extensive studies are necessary to confirm this conclusion.