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Updated: Sep 10, 2025
Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
Published on: August 18, 2022
Comparative Analysis of Reflectance Confocal Microscopy and Multiphoton Microscopy in Basal Cell Carcinoma Diagnosis
Wenwen Zhong1, Lingyang Long2, Ying Feng2
1Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Background:
Basal cell carcinoma (BCC), the most common skin malignancy, requires early diagnosis for optimal outcomes. This study evaluates the complementary roles of reflectance confocal microscopy (RCM) and multiphoton microscopy (MPM) in BCC characterization.
Methods:
Forty-seven histopathologically confirmed BCC lesions were analyzed. Preoperative dermoscopy and in vivo RCM imaging were performed; post-excision specimens underwent MPM imaging and H&E correlation.
Results:
RCM demonstrated superior visualization of tumor nests and vascular patterns, while MPM provided quantitative collagen analysis, revealing significant architectural differences between high- and low-risk subtypes (p < 0.01).
Conclusion:
The combined approach enhances diagnostic accuracy, with RCM enabling rapid assessment and MPM facilitating risk stratification through collagen metrics.
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