Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fast Reactions01:27

Fast Reactions

Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protein Surface Site Determines the Evolutionary Accessibility of Allosteric Regulation.

bioRxiv : the preprint server for biology·2026
Same author

Circulating cfDNA CD86 methylation is associated with durable response to atezolizumab-bevacizumab in hepatocellular carcinoma.

Hepatology international·2026
Same author

The mediation effects of smartphone addiction, negative emotion and psychological resilience in the relationship between social support and sleep quality among medical students.

Frontiers in psychiatry·2026
Same author

Alcohol involvement may modify the association between previous hepatitis B virus infection and hepatocellular carcinoma risk in steatotic liver disease.

Journal of gastroenterology·2026
Same author

HBsAg glycan isomer predicts on-treatment HBsAg decline in low-HBsAg chronic hepatitis B on nucleos(t)ide therapy.

Virology journal·2026
Same author

Ten simple rules for turning your qualifying exam into an NIH-style fellowship proposal: A guide for graduate students.

PLoS computational biology·2026

Related Experiment Video

Updated: May 10, 2026

Double Emulsion Generation Using a Polydimethylsiloxane PDMS Co-axial Flow Focus Device
08:58

Double Emulsion Generation Using a Polydimethylsiloxane PDMS Co-axial Flow Focus Device

Published on: December 25, 2015

16.2K

FACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments.

Samuel Thompson1, Yanrong Zhang2, Zijian Yang3

  • 1Department of Genetics, Stanford University, Stanford, CA 94305, USA.

Advanced Materials Interfaces
|August 20, 2025
PubMed
Summary

High-throughput screening of biphasic reactions is enabled by novel triple emulsion picoreactors. These microfluidic reactors, compatible with Fluorescence Activated Cell Sorting (FACS), facilitate rapid analysis of millions of picoliter reactions.

Keywords:
FACSbiphasic reactionscatalystdirected evolutiondropletsmicrofluidicsscreening

More Related Videos

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.9K
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.5K

Related Experiment Videos

Last Updated: May 10, 2026

Double Emulsion Generation Using a Polydimethylsiloxane PDMS Co-axial Flow Focus Device
08:58

Double Emulsion Generation Using a Polydimethylsiloxane PDMS Co-axial Flow Focus Device

Published on: December 25, 2015

16.2K
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.9K
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.5K

Area of Science:

  • Biochemistry
  • Chemical Engineering
  • Microfluidics

Background:

  • Biphasic reactions offer advantages for substrate solubility and catalyst activity where single solvents fail.
  • High-throughput screening of such reactions is challenging but crucial for discovery.
  • Microfluidic platforms, particularly double emulsions, provide a promising avenue for picoliter-scale reaction screening.

Purpose of the Study:

  • To develop and validate FACS-sortable triple emulsion picoreactors for screening biphasic reactions.
  • To optimize solvent phases and surfactants for stable, multi-hour biphasic reaction encapsulation.
  • To demonstrate the utility of these picoreactors for cell-free protein synthesis and enzymatic assays.

Main Methods:

  • Development of triple emulsion droplets with a fluorocarbon shell and an octanol-in-water core.
  • Screening of surfactants to ensure stability of the octanol-in-water emulsion core.
  • Encapsulation of cell-free protein synthesis and subsequent sorting using Fluorescence Activated Cell Sorting (FACS).
  • Demonstration of an in-droplet lipase assay using a fluorogenic substrate.

Main Results:

  • Stable triple emulsion picoreactors were generated, with over 70% survival after 24 hours.
  • Protein production was achieved within the biphasic core of the picoreactors.
  • Commercial FACS enabled high-throughput sorting of fluorescently labeled picoreactors at >100 Hz with high recovery rates (75-80%).
  • Successful demonstration of an in-droplet enzymatic assay for lipase activity.

Conclusions:

  • FACS-sortable triple emulsion picoreactors provide a robust platform for high-throughput screening of biphasic reactions.
  • These picoreactors are compatible with cell-free protein synthesis and enzymatic assays.
  • The technology holds potential for screening catalyst libraries, including enzymes for applications like biofuel production.