Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Attitudes, Motivation, and Predictors of Influenza Vaccination Uptake Among Primary Healthcare Professionals in Greece.

Vaccines·2026
Same author

Longitudinal Cognitive Assessment After CAR-T Cell Immunotherapy: A Prospective Cohort Study.

Cancers·2026
Same author

Disentangling verbal learning performance in multiple sclerosis: Dissociating strategy initiation and maintenance.

The Clinical neuropsychologist·2026
Same author

Effect of dexmedetomidine on cerebral homeostasis in patients undergoing craniotomy for brain tumour excision: A randomized double-blind trial.

British journal of clinical pharmacology·2026
Same author

Protective Efficacy of Selenium in Cisplatin-Induced Retinal Toxicity: An Experimental Immunohistochemical and Ultrastructural Analysis.

Nutrients·2026
Same author

Efficacy and safety of complement inhibitors and FcRn blockers in generalized AChR antibody-positive myasthenia gravis: a meta-analysis.

Journal of neurology·2026

Related Experiment Video

Updated: Jul 20, 2026

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells
15:53

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells

Published on: August 21, 2013

15.1K

Valproic Acid Causes Extensive Cell Detachment and Death in Differentiated Sh-SY5Y Cell Cultures: An In Vitro

Ioannis Dardalas1, Roza Lagoudaki2, Efstratios K Kosmidis2

  • 1Laboratory of Clinical Pharmacology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, GRC.

Cureus
|August 20, 2025
PubMed
Summary

Valproic acid (VA) causes significant cell death and detachment in differentiated neuron-like cells. This study highlights the critical need for further research into VA

Keywords:
in vitroneurotoxicitysh-sy5yvalproatevalproic acid

More Related Videos

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

28.1K
Author Spotlight: Developing Multiplexed Kinetic Assays for Organoid-Based Drug Response Analysis
09:13

Author Spotlight: Developing Multiplexed Kinetic Assays for Organoid-Based Drug Response Analysis

Published on: January 5, 2024

1.4K

Related Experiment Videos

Last Updated: Jul 20, 2026

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells
15:53

Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells

Published on: August 21, 2013

15.1K
Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

28.1K
Author Spotlight: Developing Multiplexed Kinetic Assays for Organoid-Based Drug Response Analysis
09:13

Author Spotlight: Developing Multiplexed Kinetic Assays for Organoid-Based Drug Response Analysis

Published on: January 5, 2024

1.4K

Area of Science:

  • Neuroscience
  • Pharmacology
  • Cell Biology

Background:

  • Valproic acid (VA) is a long-established medication for epilepsy, migraines, and bipolar disorder.
  • Emerging research indicates VA's potential in treating various cancers.
  • Known teratogenic effects necessitate caution, especially during pregnancy.

Purpose of the Study:

  • To investigate the dose-dependent effects of valproic acid (VA) on differentiated SH-SY5Y cell cultures.
  • To assess the impact of VA on cell morphology and viability.

Main Methods:

  • SH-SY5Y cells were differentiated into a mature neuron-like phenotype.
  • Cells were subsequently treated with valproic acid (VA) at concentrations of 1 mM and 10 mM.
  • Morphological changes and cell adherence were compared between treated and control groups.

Main Results:

  • Valproic acid (VA) treatment led to significant cell detachment and death in a dose-dependent manner.
  • A 44.0% reduction in adherent cells was observed at 1 mM VA.
  • A 95.9% reduction in adherent cells was noted at 10 mM VA.

Conclusions:

  • Valproic acid (VA) induces substantial cell detachment and death in differentiated SH-SY5Y neuronal cell cultures.
  • The observed cytotoxic effects underscore the need for further investigation into VA's impact on neuronal cells.
  • Additional research is crucial to fully understand these effects and their implications.