Related Experiment Video
Updated: Sep 8, 2025
16:41
A Protocol for Computer-Based Protein Structure and Function Prediction
Published on: November 3, 2011
68.8K
Exploring structure-function relationships in engineered receptor performance using computational structure
William K Corcoran1,2,3, Amparo Cosio1,3, Hailey I Edelstein1,3
1Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.
GEN Biotechnology
|August 20, 2025
Summary
Structural modeling tools can predict engineered receptor performance. This study used these tools to analyze cytokine receptors, finding structural features explain functional variations, guiding future synthetic receptor design.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- Engineered receptors are crucial for cell-based therapies.
- Understanding the structural basis of engineered receptor performance is limited.
- Protein structure prediction tools offer new analytical possibilities.
Purpose of the Study:
- To investigate if predicted structural features explain functional variations in engineered receptors.
- To explore the utility of post hoc structural modeling in receptor design.
- To assess the role of structural mechanisms in engineered receptor performance.
Main Methods:
- Utilized advanced protein structure prediction tools for post hoc analysis.
- Modeled a library of engineered receptors derived from natural cytokine receptors.
- Quantified structural features to correlate with observed receptor performance.
Main Results:
- Predicted structural features explained significant variation in engineered receptor performance for a subset of cases.
- Observed trends in structural-performance correlations were consistent across diverse receptor sets.
- Demonstrated the potential of structural modeling to elucidate functional differences.
Conclusions:
- Protein structure prediction tools can provide insights into engineered receptor function.
- Structural features are key determinants of engineered receptor performance.
- Structure prediction-guided engineering holds promise for advancing cell-based therapies.
Keywords:
Engineered receptorsprotein structure predictionstructure-function relationshipsynthetic biologyMore Related Videos
05:08Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
Published on: July 8, 2025
328
06:50Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
Published on: January 26, 2024
2.0K
Related Concept Videos
01:28Structure-Activity Relationships and Drug Design
1.0K
Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
1.0K
01:15G Protein-coupled Receptors
13.2K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
13.2K
01:29The Two-State Receptor Model
2.4K
The two-state receptor model explains a drug's interaction with receptors, such as G protein-coupled receptors and ligand-gated ion channels, to induce or inhibit a biological response. When no natural ligands are present, a receptor exists in an equilibrium of inactive (Ri) and active (Ra) conformations. The inactive form does not produce a response, while the active form generates a basal effect known as constitutive activity.
The binding affinity of a drug determines its interaction with...
The binding affinity of a drug determines its interaction with...
2.4K
01:49Conserved Binding Sites
4.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.3K
02:40Ligand Binding Sites
13.1K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
13.1K
01:24Protein Organization
7.0K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
The primary structure of a protein is its amino acid sequence....
7.0K