Protocol for generation and utilization of patient-derived organoids from multimodal specimen
Sumin Kang1, Mi Rim Lee2, Wonyoung Choi3
1Molecular Imaging Branch, Division of Convergence Technology, Research Institute of National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang, Gyeonggi-do 10408, Republic of Korea; Department of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, 323 Ilsan-ro, Ilsandong-gu, Goyang, Gyeonggi-do 10408, Republic of Korea.
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Patient-derived organoids (PDOs) are robust preclinical models for precision oncology. However, most existing protocols depend on surgically resected specimens, limiting their applicability to patients who are ineligible for surgery. Here, we present a protocol for generating PDOs from various clinically accessible specimens-including biopsies (endoscopic ultrasound-guided fine needle biopsy [EUS-FNB], percutaneous liver biopsy [PLB], ascites, and pleural effusion)-across cancer types. We describe steps for specimen transport, tumor cell isolation, culture, biobanking, and high-throughput drug screening, supporting reproducible PDO applications in translational research across diverse clinical settings. For complete details on the use and execution of this protocol, please refer to Lee et al.1.
