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Updated: Sep 10, 2025
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
Published on: September 7, 2022
Elevated interleukin-10, -22, -24, and -26 in serum samples of children with infectious mononucleosis
Dalei Li1, Kangwei Mao1, Peiliang Luo1
1The Affiliated Lianyungang Hospital of Xuzhou Medical University (the First People's Hospital of Lianyungang, the First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang Clinical College of Nanjing Medical University), Department of Pediatrics, Lianyungang, Jiangsu, China.
Background:
Infectious mononucleosis (IM) is an infectious disease with different stages of onset, and the pathogenesis of IM remains unclear. This study aimed to investigate the levels of interleukin (IL)-10 family cytokines IL-10, -22, -24, and -26 in serum samples of patients with infectious mononucleosis (IM) and the potential diagnostic values.
Methods:
Thirty children with IM were enrolled in the study, and serum samples were collected; 30 healthy children served as the controls. Serum samples from the patients and healthy controls were collected, and IL-10, -22, -24, and -26 were determined by the ELISA method. An automatic biochemical analyser detected the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Moreover, potential diagnostic values of IL-10, -22, -24 and -26 have been analysed using ROC curve analysis, and the correlation between the levels of IL-10, -22, -24 and -26 in patients was analysed. Correlation between IL-10, -22, -24, and -26 and ALT and AST were analysed.
Results:
IL-10, -22, -24, and -26 levels increased in serum samples of patients with IM compared to healthy controls. ROC analysis showed that the AUC of IL-10, -22, -24, and -26 were 0.8500, 0.7078, 0.8056, and 0.9167, respectively, suggesting that IL-10, -22, -24, and -26 were good biomarkers. Moreover, IL-10, -22, -24 and -26 levels were positively correlated with the ALT and AST of the patients.
Conclusions:
IL-10, -22, -24, and -26 were upregulated in the serum of patients with IM, suggesting they may function as potential biomarkers.
