Protocol for synchronizing waveforms and videos from electropenetrography studies of arthropods

Alternating current-direct current (AC-DC) electropenetrography (EPG) is a non-invasive approach for quantifying parasitic arthropods' probing and ingestion behaviors inside host tissues. Here, we present a procedure for using the Observer XT behavioral coding software to synchronize EPG waveforms with video recordings. We describe the steps for performing, importing, and analyzing simultaneous recordings. This protocol has potential applications for the initial correlation of waveforms with arthropod biological activities. For complete details on the use and execution of this protocol, please refer to Cooper et al.¹.