Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

UV-induced structural changes in chromatin.

H Lang, Vengerov YuYu, C Zimmer

    Biomedica Biochimica Acta
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    FKBP5 expression is related to risky foraging in house sparrows.

    Hormones and behavior·2026
    Same author

    Divergent climate impacts despite similar response to temperature in a widespread aerial insectivore.

    bioRxiv : the preprint server for biology·2025
    Same author

    Differentially spliced mitochondrial CYP419A1 contributes to ethiprole resistance in Nilaparvata lugens.

    Insect biochemistry and molecular biology·2025
    Same author

    Screening H3 Histone Acetylation in a Wild Bird, the House Sparrow (<i>Passer Domesticus</i>).

    Integrative organismal biology (Oxford, England)·2024
    Same author

    Thoracic impedance pneumography in propofol-sedated patients undergoing percutaneous endoscopic gastrostomy (PEG) placement in gastrointestinal endoscopy: A prospective, randomized trial.

    Journal of clinical anesthesia·2024
    Same author

    Reconstruction of the Corticospinal Tract in Patients with Motor-Eloquent High-Grade Gliomas Using Multilevel Fiber Tractography Combined with Functional Motor Cortex Mapping.

    AJNR. American journal of neuroradiology·2023
    Same journal

    Intracerebroventricular administration of hypertonic sodium chloride solution reduces the sensitivity of the baroreceptor heart reflex in anaesthetized rats.

    Biomedica biochimica acta·1991
    Same journal

    An efficient chemical-enzymatic synthesis of LHRH N-terminal pentapeptide.

    Biomedica biochimica acta·1991
    Same journal

    Adrenergic cardiovascular actions in rats as affected by dichloromethane exposure.

    Biomedica biochimica acta·1991
    Same journal

    Lipoxygenase-inhibitory action of antiviral polymeric oxidation products of polyphenols.

    Biomedica biochimica acta·1991
    Same journal

    Uptake of lysine and incorporation into proteins of the cortex during a short-term hypoxia of neonatal rats.

    Biomedica biochimica acta·1991
    Same journal

    Influence of intestinal resection, type of dietary fat and time on the digestive and metabolic utilization of fat in rats.

    Biomedica biochimica acta·1991
    See all related articles

    UV radiation causes significant structural changes in chromatin, even at low doses. DNA within chromatin is more vulnerable to UV damage and strand breaks than free DNA.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Photobiology

    Background:

    • Chromatin, the complex of DNA and proteins that forms chromosomes, is susceptible to environmental damage.
    • Ultraviolet (UV) radiation is a known mutagen and can induce DNA damage.
    • Understanding UV-induced chromatin alterations is crucial for cell survival and disease research.

    Purpose of the Study:

    • To investigate the structural changes in chromatin induced by UV irradiation.
    • To determine the effects of UV radiation on DNA integrity within chromatin.
    • To compare the sensitivity of chromatin-bound DNA versus free DNA to UV damage.

    Main Methods:

    • Circular Dichroism (CD) spectroscopy
    • Electron Microscopy (EM)
    • Gel Electrophoresis

    Related Experiment Videos

    Main Results:

    • Chromatin undergoes significant structural alterations upon UV exposure, transitioning from a higher-ordered to an unfolded state even at low fluences.
    • Histone H1 is lost only at higher UV fluences (>10 kJ/m²), while core histones remain largely unchanged in the low fluence range.
    • DNA within chromatin exhibits increased sensitivity to UV-induced strand scission compared to free DNA, with fragmentation occurring at 5.5 kJ/m² in chromatin versus 15 kJ/m² in free DNA.

    Conclusions:

    • UV radiation induces substantial structural instability in chromatin at low doses.
    • Chromatin structure offers limited protection and can even sensitize DNA to UV-induced strand breaks.
    • The findings highlight the vulnerability of genomic organization to UV damage, with implications for cellular response and potential mutagenic outcomes.