[Activity of urease in microcapsules].

Area of Science:

• Biochemistry
• Materials Science
• Chemical Engineering

Context:

• Urease enzyme activity is crucial in various biological and industrial processes.
• Enzyme immobilization is a key technique for enhancing enzyme stability and reusability.
• Uremia poses significant health challenges, necessitating effective detoxification strategies.

Purpose:

• To investigate the activity and stability of urease immobilized in cellulose sulphate microcapsules.
• To compare the performance of encapsulated urease with free urease in solution.
• To evaluate the potential of this immobilized urease system for therapeutic applications in uremia.

Summary:

• Urease was immobilized within microcapsules formed by cellulose sulphate and polydimethyldiallyl ammonium chloride.
• Both free and encapsulated urease demonstrated similar pH optima.
• Immobilization led to a 35-38% decrease in urease activity, but significantly improved long-term stability compared to free urease.

Impact:

• The developed urease immobilization technique offers a promising approach for enhanced enzyme stability.
• Improved stability of encapsulated urease could be beneficial for applications requiring sustained enzymatic activity.
• This method holds potential for developing active detoxification therapies for uremia.