Bioactive peptide based supramolecular hydrogel as an efficient drug/cell delivery platform for ophthalmic...

Area of Science:

• Biomaterials Science
• Ophthalmology
• Nanotechnology

Background:

• Developing advanced drug delivery systems is crucial for effective ophthalmic treatments.
• Peptide-based supramolecular hydrogels offer unique properties for biomedical applications.

Purpose of the Study:

• To create a bioactive peptide-based supramolecular hydrogel for ophthalmic cell and drug delivery.
• To evaluate the hydrogel's stability, cell proliferation support, drug encapsulation, and therapeutic efficacy in a bacterial keratitis model.

Main Methods:

• Synthesis and characterization of the NapFFGGRGD amphiphile and its self-assembly into a hydrogel.
• In vitro evaluation of hydrogel stability and support for human corneal epithelial cells (HCECs).
• In vivo assessment of vancomycin-loaded hydrogel efficacy in a rat model of bacterial keratitis.

Main Results:

• The NapFFGGRGD amphiphile successfully formed a stable supramolecular hydrogel.
• The hydrogel demonstrated excellent in vitro stability and supported HCEC proliferation.
• Encapsulated hydrophobic and hydrophilic drugs retained their activity.
• Vancomycin-loaded hydrogel effectively treated bacterial keratitis by eliminating bacteria and promoting corneal healing.

Conclusions:

• The NapFFGGRGD supramolecular hydrogel is a promising platform for ophthalmic applications.
• It enables efficient delivery of both cells and drugs.
• The hydrogel shows significant therapeutic potential for treating eye infections and promoting tissue regeneration.