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Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

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In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
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Related Experiment Video

Updated: Sep 10, 2025

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
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Single-cell transcriptomic landscape of sciatic nerve after transection injury.

Yiben Ouyang1,2, Mingqian Yu1, Tieyuan Zhang2,3

  • 1School of Medicine, Nankai University, No. 94, Weijin Road, Nankai District, Tianjin, 300071, PR China.

Journal of Neuroinflammation
|August 24, 2025
PubMed
Summary

Peripheral nerve injuries hinder recovery. This study maps cellular changes after sciatic nerve injury using single-cell sequencing, revealing key immune and glial cell roles and potential therapeutic targets for improved nerve regeneration.

Keywords:
TGF−β signalingGlial cell reprogrammingMacrophage heterogeneityNerve regenerationNeurofibroblast–glia interactionSciatic nerve transectionSingle−cell RNA−seq

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An In Vivo Murine Sciatic Nerve Model of Perineural Invasion
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Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Peripheral nerve injuries often lead to poor functional recovery due to limited regeneration.
  • Understanding the cellular dynamics post-injury is crucial for developing effective treatments.

Purpose of the Study:

  • To create a detailed, time-resolved single-cell atlas of peripheral nerve regeneration after sciatic nerve transection.
  • To identify key cellular players and molecular signaling pathways involved in nerve repair.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) of rat sciatic nerve tissue at seven time points post-transection.
  • Unsupervised clustering to identify cell types and pseudotime analysis for dynamic trajectories.
  • Cell-cell communication analysis and bulk transcriptomic validation.

Main Results:

  • Identified dynamic changes in neurofibroblasts, glial cells, immune cells, and vascular cells during regeneration.
  • Revealed early macrophage and granulocyte infiltration, followed by fibroblast and Schwann cell proliferation.
  • Uncovered critical collagen and PTN signaling pathways and sustained TGF-β activation.

Conclusions:

  • Provides a comprehensive single-cell map of peripheral nerve regeneration.
  • Defines the immune-neurofibroblast-glial cell axis regulatory circuits.
  • Identifies phase-specific therapeutic targets to enhance functional recovery after severe nerve injury.