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Related Experiment Video

Updated: Sep 10, 2025

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional 3D Model

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Published on: June 11, 2014

Coupled Dynamics in Phenotype and Tissue Spaces Shape the Three-Dimensional Cancer Invasion.

Austin Naylor¹, Maximilian Libmann¹, Izabel Raab²

¹Department of Physics, Oregon State University, Corvallis, Oregon 97331, USA.

|August 25, 2025

Summary

This summary is machine-generated.

Cancer cells invade tissues by changing their behavior and movement. This study reveals how breast cancer cells remodel their 3D environment, leading to specific cell phenotypes and motility patterns during invasion.

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Last Updated: Sep 10, 2025

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional 3D Model

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Published on: June 11, 2014

Modeling and Imaging 3-Dimensional Collective Cell Invasion

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Published on: December 7, 2011

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

Area of Science:

Biophysics
Cancer Biology
Cellular Mechanics

Background:

Metastasis involves cancer cell migration through complex 3D tissue environments.
Tumor invasiveness is linked to cancer cell migration dynamics.
The interplay between cancer cells and their microenvironment is crucial for metastasis.

Purpose of the Study:

To investigate tumor spheroid invasion in 3D collagen matrices.
To understand cancer cell phenotype transitions and motility during invasion.
To explore the impact of microenvironment remodeling on cell migration.

Main Methods:

Quantitative experiments with breast cancer cell spheroids in 3D collagen.
Artificial intelligence (AI)-driven image processing for cell behavior analysis.
Mathematical modeling to describe invasion dynamics and phenotype changes.

Main Results:

Observed rapid cancer cell phenotype transitions and phenotype-dependent motility.
Demonstrated that persistent invasion remodels the extracellular matrix (ECM).
Identified filopodial cells as the predominant phenotype across different ECM conditions.

Conclusions:

Cancer cell invasion involves complex mesoscale dynamics within 3D matrices.
Cell migration phenotype plasticity and ECM remodeling are key factors in invasion.
Findings provide insights into the interplay between cancer cells and their microenvironment during metastasis.