Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chemotactic Gold Nanozyme-Powered Flasklike Pentosan Nanobots for Tumor-Specific Drug Delivery.

Advanced healthcare materials·2026

Same author

Low-temperature hyperbolic phonon polaritonics with suppressed phononic scattering.

Science advances·2026

Same author

Microalgae Oil Attenuates Liver Fat Deposition in NAFLD via Modulation of Anti-Lipogenic Genes and Insulin Signaling Pathways in HFD Mice.

Food science & nutrition·2026

Same author

Structural basis for LMBD1-dependent trafficking and cobalamin export of ABCD4.

Nature communications·2026

Same author

Research on comprehensive drought index prediction model based on CNN-LSTM.

Scientific reports·2026

Same author

Microalgae Oil Improves Hepatic Lipid Metabolism in A High-Fat Diet-Induced Mouse Model.

Journal of visualized experiments : JoVE·2026

Search research articles

Related Experiment Video

Updated: Sep 10, 2025

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Using High-Throughput Screening to Identify Crosslinking Peptides That Control Cell-Mediated Matrix Degradation.

Yingjie Wu¹, Samuel J Rozans¹, Abolfazl Salehi Moghaddam¹

¹Lehigh University, 124 E. Morton Street, Bethlehem, Pennsylvania, 18015, United States of America.

Advanced Healthcare Materials

|August 25, 2025

Summary

This summary is machine-generated.

Researchers developed a new peptide, KLVADLMASAE, for hydrogels that mimics cell migration without bulk degradation. This peptide offers improved mechanical properties and reduced degradation compared to traditional methods, enhancing cell viability and spreading.

Keywords:

cell‐matrix interactions endothelial cells high‐throughput hydrogels mesenchymal stem cells proteases

More Related Videos

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Published on: January 22, 2019

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

Related Experiment Videos

Last Updated: Sep 10, 2025

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

Published on: January 22, 2019

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

Area of Science:

Biomaterials Science
Cell Biology
Protease Engineering

Background:

Cells remodel the extracellular matrix (ECM) using proteases for migration.
Protease-degradable peptide crosslinks in hydrogels mimic ECM remodeling but often cause bulk degradation.
Existing crosslinkers like PanMMP lead to significant hydrogel degradation, altering mechanical properties.

Purpose of the Study:

To identify and optimize peptides specifically cleaved by membrane-type proteases for improved hydrogel applications.
To develop a method for discovering new protease-degradable peptides that minimize bulk hydrogel degradation.
To enhance cell migration and viability in engineered matrices.

Main Methods:

Coupled proteomic identification of candidate peptides with mass spectrometry-based functional assays.
Developed a split-and-pool synthesis approach to generate and screen over 300 peptide variants.
Utilized cell spreading and viability assays with endothelial and stem cells.
Compared degradation and mechanical properties of KLVADLMASAE-crosslinked hydrogels with PanMMP hydrogels.

Main Results:

Identified and optimized the peptide KLVADLMASAE for selective cleavage by membrane-type proteases.
KLVADLMASAE hydrogels exhibited reduced degradation by soluble proteases compared to PanMMP hydrogels.
Cells cultured in KLVADLMASAE hydrogels showed comparable spreading and viability to PanMMP hydrogels.
KLVADLMASAE hydrogels maintained stiffness and showed less macroscopic degradation over 14 days.

Conclusions:

The developed high-throughput approach successfully identified and optimized a novel peptide for controlled ECM degradation in hydrogels.
KLVADLMASAE represents a promising alternative to existing crosslinkers, offering improved matrix stability and cell compatibility.
This strategy enables the design of biomaterials with tunable degradation profiles for advanced tissue engineering and cell-based assays.