Chemotactic Gold Nanozyme-Powered Flasklike Pentosan Nanobots for Tumor-Specific Drug Delivery

Enzyme-powered nanobots represent a promising strategy for active and targeted drug delivery due to their autonomous propulsion and directional motion. However, the instability and environmental vulnerability of natural enzymes severely compromise their functionality in the tumor microenvironment (TME). Herein, we report a chemotactic nanobot system powered by gold nanozymes and constructed within a flasklike pentosan architecture for tumor-specific drug delivery. Benefiting from the intrinsic glucose oxidase-like and peroxidase-like activities of gold nanozymes, these nanobots exploit endogenous glucose and elevated hydrogen peroxide (H₂O₂) levels in the TME to achieve sustained propulsion, chemotactic migration along H₂O₂ gradients, enhanced tumor penetration, and microenvironment-responsive drug release. In vivo studies demonstrate a 4.7-fold increase in tumor accumulation and a tumor growth inhibition rate of 75.8%, significantly outperforming free doxorubicin treatment. This study presents a nanozyme-powered chemotactic nanobot platform enabling dynamic navigation of tumor biochemical gradients and precise cancer chemotherapy.