Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Covalent Programming at DNA Base-Pairing Interfaces.

Journal of the American Chemical Society·2026
Same author

Fluorogenic oligonucleotide cleavage probes with a branched linker improve RNA detection.

Nucleic acids research·2025
Same author

"Click" disaggregation-induced emission of a fluorescent dye.

Chemical communications (Cambridge, England)·2025
Same author

Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication.

Science advances·2024
Same author

Fluorescent molecular rotors detect <i>O</i><sup>6</sup>-methylguanine dynamics and repair in duplex DNA.

Chemical communications (Cambridge, England)·2024
Same author

Formal [4 + 2] Cycloadditions of Maleimides on Duplex DNA.

Bioconjugate chemistry·2023

Related Experiment Video

Updated: Sep 10, 2025

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

17.1K

A Fluorogenic Hydrazino-Pictet-Spengler Reaction in Live Cells.

Kaleena Basran1, Nathan W Luedtke1,2

  • 1Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montréal, Québec, H3A 0B8, Canada.

Angewandte Chemie (International Ed. in English)
|August 25, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new fluorescent probe, FLipA-HIPS, for detecting lipidic aldehydes in live cells. This probe offers a sensitive and selective method for visualizing oxidative stress markers within cellular environments.

Keywords:
AldehydesBioimagingFluorogenic reactionsLipidsOxidative stress

More Related Videos

Imaging Protein-protein Interactions in vivo
11:15

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

21.4K
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.1K

Related Experiment Videos

Last Updated: Sep 10, 2025

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

17.1K
Imaging Protein-protein Interactions in vivo
11:15

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

21.4K
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.1K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Chemical Biology

Background:

  • Lipid peroxidation produces aldehydic lipids linked to oxidative stress and cellular signaling.
  • Current methods lack the ability to detect these lipidic aldehydes in live cells.

Purpose of the Study:

  • To develop a novel fluorogenic probe for detecting lipidic aldehydes in live cells.
  • To characterize the probe's reactivity and fluorescence properties.

Main Methods:

  • Development of a fluorogenic hydrazino-Pictet-Spengler (HIPS) probe named FLipA-HIPS.
  • In vitro characterization of probe reactivity with various aldehydes.
  • Live-cell imaging using confocal microscopy to detect lipidic aldehydes.

Main Results:

  • FLipA-HIPS is a nonfluorescent probe that becomes highly fluorescent upon reaction with aldehydes.
  • The probe exhibits enhanced fluorescence in lipid environments (SDS micelles) compared to aqueous solutions.
  • FLipA-HIPS demonstrates selective and rapid staining of lipidic aldehydes in live cells, co-localizing with the endoplasmic reticulum.

Conclusions:

  • The FLipA-HIPS probe provides a sensitive and selective tool for detecting lipidic aldehydes in live cells.
  • The hydrazino-Pictet-Spengler reaction shows promise for developing new aldehyde biosensors.
  • This methodology facilitates the study of oxidative stress and signaling pathways involving lipidic aldehydes in cellular contexts.