Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.6K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.6K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.5K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Detection of Thromboembolism during Open Repair for Complex Aortoiliac Aneurysms Using Continuous Noninvasive Lower Limb Oximetry.

Annals of vascular diseases·2026
Same author

Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.

Nature communications·2026
Same author

Left Ventricular Venting Using a Transaortic Pigtail Catheter in Pediatric Myocarditis Requiring Extracorporeal Membrane Oxygenation.

ASAIO journal (American Society for Artificial Internal Organs : 1992)·2026
Same author

Staged repair of the anomalous origin of the right pulmonary artery from the ascending aorta with aortic coarctation.

Annals of pediatric cardiology·2026
Same author

Stress-induced CXCL13 regulates pancreatic exocrine homeostasis, age-related chronic inflammation, and cancer progression.

Science advances·2026
Same author

AMPK-p38 axis converts human pluripotent stem cells to naive state.

iScience·2026

Related Experiment Video

Updated: Apr 12, 2026

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
06:47

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

10.9K

An iPSC-based in vitro model recapitulates human thymic epithelial development and multi-lineage specification.

Yann Pretemer1, Yuxian Gao1,2, Kaho Kanai1,3

  • 1Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.

Nature Communications
|August 25, 2025
PubMed
Summary

Researchers developed a human induced pluripotent stem cell (iPSC) model for thymus organogenesis. This model generates diverse thymic epithelial cell (TEC) lineages in vitro, aiding T cell development research and potential treatments for congenital thymic disorders.

More Related Videos

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

7.3K
Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids
03:31

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids

Published on: October 4, 2024

1.8K

Related Experiment Videos

Last Updated: Apr 12, 2026

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
06:47

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

10.9K
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

7.3K
Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids
03:31

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids

Published on: October 4, 2024

1.8K

Area of Science:

  • Immunology
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Thymic epithelial cells (TECs) are vital for T cell development but challenging to study in humans due to heterogeneity and isolation difficulties.
  • Understanding the differentiation pathways of diverse TEC lineages from common progenitors is crucial but remains poorly understood.

Purpose of the Study:

  • To establish a human induced pluripotent stem cell (iPSC)-based model for in vitro thymus organogenesis.
  • To investigate the differentiation trajectories of various TEC lineages and their role in T cell development.

Main Methods:

  • Controlled retinoid signaling and self-directed differentiation of human iPSCs to generate TEC progenitor-like and mature TEC populations.
  • Co-culture of induced TECs with thymocytes to assess their function in T cell generation and maturation.
  • Characterization of TEC subpopulations, including MHCII+, AIRE+, and mimetic TECs.

Main Results:

  • Successfully derived FOXN1+ TEC progenitor-like cells and mature MHCII+ TECs resembling cortical and medullary subtypes in vitro.
  • Induced TECs supported the generation of naive T cells with diverse T cell receptor (TCR) repertoires.
  • Demonstrated in vitro differentiation into AIRE+ and mimetic TEC subpopulations.

Conclusions:

  • The developed iPSC model enables comprehensive in vitro study of human TEC differentiation from specification to maturation.
  • Provides novel insights into human thymus development and offers potential for regenerative medicine in congenital thymic disorders.