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Quantitative comparison and current issues in starch granule-associated proteins removal methods: A mini review
Xindong Xu1, Yuhuan Geng2, Ji Ma2
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, Foshan University, Foshan 528225, China; China-Singapore International Joint Research Institute, Guangzhou 510700, China.
This review compares methods for removing starch granule-associated proteins (SGAP), crucial for starch properties. It quantifies removal effectiveness and suggests improvements for accurate assessment and sustainable future methods.
Area of Science:
- Food Science
- Biochemistry
- Materials Science
Background:
- Starch granule-associated proteins (SGAP) influence starch physicochemical properties.
- Existing SGAP removal methods lack systematic quantitative and qualitative comparison.
Purpose of the Study:
- To systematically compare SGAP removal methodologies.
- To address the absence of quantitative and qualitative assessments of SGAP removal techniques.
- To propose solutions for accurate assessment and future SGAP removal strategies.
Main Methods:
- Compiled and analyzed research on SGAP removal from the past five years.
- Quantified SGAP removal effectiveness by measuring protein content decrease.
- Reviewed challenges in assessing removal efficiency and residual SGAP.
Main Results:
- Compared effectiveness of SDS, alkali, and protease treatments for SGAP removal.
- Identified issues like overestimation of removal efficiency and residual SGAP assessment.
- Recommended low-temperature nitrogen adsorption/desorption and Raman spectroscopy/microscopy for evaluation.
Conclusions:
- Existing SGAP removal methods have limitations such as reagent residue, long processing times, and sustainability concerns.
- Future research should focus on developing physical removal methods leveraging SGAP electrostatic properties.
- Accurate assessment methods are crucial for optimizing SGAP removal processes.
