Ultrasound Contrast Agents in Mice: Bridging the Gap from Preclinical Research to Clinical Translation.

Area of Science:

• Preclinical Imaging
• Medical Ultrasound
• Murine Models

Background:

• Contrast-enhanced ultrasound (CEUS) is a valuable non-invasive imaging technique for preclinical research.
• Current lack of standardized protocols for CEUS in mice hinders reproducibility and translatability.
• CEUS offers advantages like real-time imaging and no ionizing radiation.

Purpose of the Study:

• To present a dedicated, standardized protocol for performing CEUS in murine models.
• To improve the reliability and reproducibility of CEUS in preclinical research.
• To facilitate the translation of findings from mouse studies to clinical applications.

Main Methods:

• Development of a comprehensive CEUS workflow protocol for mice.
• Detailed guidelines cover contrast agent preparation, animal handling, and administration.
• Specific recommendations for CEUS imaging parameters are included.

Main Results:

• A standardized CEUS protocol for mice has been established.
• The protocol addresses the full workflow from agent preparation to imaging.
• Guidelines aim to enhance best practices in preclinical CEUS.

Conclusions:

• Standardized CEUS protocols are crucial for reproducible preclinical research in mice.
• This protocol enhances the effectiveness and translatability of CEUS findings.
• Adoption of this workflow can accelerate disease characterization and therapeutic development.