All-in-one Biocomputing Nanoagents with Multilayered Transformable Architecture based on DNA Interfaces.

Area of Science:

• Biotechnology
• Nanotechnology
• Molecular Biology

Background:

• Pathogen diversity necessitates advanced prevention mechanisms.
• Intelligent agents with molecular logic offer theranostic potential.
• Existing nanoagents require further development for full theranostic capabilities.

Purpose of the Study:

• To extend nanoagent technology using nucleic acid interfaces.
• To design nanostructures implementing Boolean logic gates.
• To create autonomous biocomputing agents for theranostics.

Main Methods:

• Utilized "Biocomputing based on particle disassembly" technology.
• Developed nanoparticle platforms with DNA interfaces for logic gates.
• Investigated nanostructure performance with various output detection formats.

Main Results:

• Demonstrated autonomous theranostic biocomputing agents ("DNA-transformers").
• Achieved a complete set of Boolean logic gates (YES, NOT, AND, OR) on a single platform.
• Showcased programmed outputs for theranostic applications, including cancer cell targeting.

Conclusions:

• Developed all-in-one DNA-based nanoagents advance molecular logic devices.
• Established a versatile platform for smart nanoagents.
• The platform is suitable for both diagnostic and therapeutic applications.