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Updated: Sep 10, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
Published on: May 14, 2020
METTL5-mediated 18S rRNA m6A modification enhances ribosome assembly and ABA response in Arabidopsis
Ping Li1, Yu Zhang1, Songyao Zhang2
1Temasek Life Sciences Laboratory National University of Singapore Singapore Singapore.
Abstract:
METTL5 catalyzes the N 6-methyladenosine (m6A) methylation at A1771 in 18S rRNA, a modification essential for its association with the ribosomal protein RPL24A, facilitating the assembly of 80S ribosome. This facilitates the translation of mRNAs encoding the detoxifying glutathione S-transferase (GST) enzymes, thereby maintaining normal reactive oxygen species (ROS) levels and ensuring proper abscisic acid (ABA) responses. In mettl5 mutants, the absence of m6A1771 compromises RPL24A incorporation and ribosome assembly, impairing the translation of GSTs. This results in ROS excessive accumulation and hypersensitivity to ABA.
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