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Updated: Apr 17, 2026

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
Published on: May 14, 2020
Emerging roles of non-m6A mRNA modifications in plants
Zhenfeng Teng1, Lisha Shen2,3
1Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
None:
Internal chemical modifications of mRNA constitute a key epitranscriptomic layer of gene regulation in eukaryotes. Although N6-methyladenosine (m6A) has been the most intensively studied, accumulating evidence reveals important roles of non-m6A mRNA modifications, such as 5-methylcytosine, N4-acetylcytidine and pseudouridine, in plants. These modifications modulate diverse aspects of mRNA metabolism, including alternative splicing, stability, translation and long-distance transport, and thereby shape plant development and environmental adaptation. Here we summarize current advances in understanding non-m6A mRNA modifications in plants, emphasizing their regulatory roles in mRNA metabolism and their effects on plant development and stress resilience. We also review the detection technologies for non-m6A modifications and discuss key challenges and future directions towards elucidating their regulatory functions.
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