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Updated: Sep 10, 2025

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
Antihyperglycemic drug screening: 4-nitrophenol intermittent pulse amperometry as a convenient α-glucosidase
Waswan Prempinij1, Albert Schulte1
1School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wang Chan Valley Rayong 21210 Thailand albert.s@vistec.ac.th 0066 (0) 873 758 518.
Abstract:
We present intermittent pulse amperometry (IPA) as a practical direct electrochemical readout option in a 4-nitrophenol (4-NP)-assisted α-glucosidase (α-GL) activity assay. The feasibility of the strategy was validated through proof-of-principle inhibition tests on a selection of gold-standard α-GL inhibitors and commercial inhibitor-containing plant-based food supplements, and the quality of the obtained results confirmed well the potential of this method in the identification of new antidiabetic drugs. The proposed IPA/4-NP-based α-GL activity inhibition assay is an easy-to-establish user-friendly approach for the rapid and economical detection of inhibitor candidates within small to medium-sized sample libraries and is intended to complement, not replace, other currently used methods and to valuably widen the analytical scheme set for applicants in the academic and industrial pharma sectors.
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