A New Approach for Interferent-Free Amperometric Biosensor Production Based on All-Electrochemically Assisted...

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Amperometric enzyme electrodes are crucial for biosensing.
• Existing methods for enzyme immobilization and transducer modification face challenges in selectivity and stability.
• Interference from common sample components affects biosensor accuracy.

Purpose of the Study:

• To develop a novel, fully electrochemical approach for producing amperometric enzyme electrodes.
• To improve enzyme immobilization and transducer selectivity for enhanced biosensor performance.
• To investigate the coupling of electrophoretic protein deposition and electrosynthesis of permselective polymers.

Main Methods:

• Enzyme (glucose oxidase) immobilization via in situ co-crosslinking using electrophoretic protein deposition.
• Electrosynthesis of non-conducting polymers with built-in permselectivity to create a protective film.
• Coupling of these two electrochemical techniques and evaluation of different polymer combinations.

Main Results:

• Successful fabrication of glucose oxidase biosensors using a fully electrochemical approach.
• Demonstrated improvement in selectivity and rejection of common interferents.
• Achieved good analytical performance and low bias in glucose measurements from serum samples.

Conclusions:

• The developed all-electrochemical method offers an easy fabrication process for biosensors.
• The approach allows for controlled enzyme immobilization and thickness.
• Results indicate potential for interferent-free transduction and device miniaturization.