Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of ER in the Secretory Pathway01:17

Role of ER in the Secretory Pathway

5.6K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.6K
Stringent Response in E. coli01:23

Stringent Response in E. coli

52
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
52
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

64
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
64
Global Regulatory Systems01:28

Global Regulatory Systems

71
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
71
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

77
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
77

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Chemotherapy and Radiotherapy on Iris Metastases: A Case Report.

Ocular oncology and pathology·2026
Same author

Ocular Safety Signals of Fibroblast Growth Factor Receptor Inhibitors in Postmarketing Surveillance.

American journal of ophthalmology·2026
Same author

<sup>68</sup>Ga-FAPI-46 PET/CT Versus <sup>18</sup>F-FDG PET/CT for Pancreatic Cancer: A Feasibility Study.

Journal of medical imaging and radiation oncology·2026
Same author

1-2-3-4 Sign FDG PET Sign in Sarcoidosis.

Journal of medical imaging and radiation oncology·2026
Same author

Immune Mediators in Birdshot Chorioretinopathy: A Systematic Review.

Ocular immunology and inflammation·2026
Same author

Green-synthesized CdTe quantum dots: dual-action nanomaterials tackling antimicrobial resistance and cancer.

RSC advances·2026

Related Experiment Video

Updated: Sep 10, 2025

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
09:12

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Published on: November 14, 2018

8.0K

Role of Endoplasmic Reticulum Stress-Associated Genes in Septic Neonatal Foals.

Dipak Kumar Sahoo1, David Wong1, Biswaranjan Paital2

  • 1Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.

Antioxidants (Basel, Switzerland)
|August 28, 2025
PubMed
Summary

Sepsis in foals triggers oxidative stress and endoplasmic reticulum (ER) stress, altering gene expression. Targeting ER stress may offer new therapeutic and prognostic strategies for neonatal foal sepsis.

Keywords:
endoplasmic reticulum stresshorseinflammationoxidative stresssepsissignaling pathways

More Related Videos

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings
14:40

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

9.5K
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
08:46

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

6.5K

Related Experiment Videos

Last Updated: Sep 10, 2025

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
09:12

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Published on: November 14, 2018

8.0K
Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings
14:40

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Published on: October 25, 2015

9.5K
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
08:46

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

6.5K

Area of Science:

  • Veterinary Medicine
  • Molecular Biology
  • Pathogenesis of Sepsis

Background:

  • Sepsis involves complex inflammation and oxidative stress (OS), particularly in neonatal foals with compromised antioxidant defenses.
  • Oxidative stress can impair redox-sensitive organelles like the endoplasmic reticulum (ER), disrupting cellular signaling pathways crucial for sepsis progression.
  • Endoplasmic reticulum stress disorder is implicated in the pathogenesis of sepsis, affecting redox balance, inflammation, and apoptosis.

Purpose of the Study:

  • To investigate the relationship between oxidative stress conditions during sepsis and the gene expression of endoplasmic reticulum (ER) stress markers in neonatal foals.
  • To identify specific ER stress-responsive genes that are altered in septic foals and correlate with disease severity and antioxidant enzyme activity.

Main Methods:

  • Blood samples were collected from healthy and septic neonatal foals.
  • RNA extraction and RNA sequencing were performed to analyze gene expression profiles.
  • Correlation analyses were conducted between gene expression, sepsis scores, and antioxidant enzyme activities.

Main Results:

  • The ER stress pathway was activated in septic foals, with several ER stress-associated genes (e.g., CLU, BCL2L1, USP14, BFAR, OPA1) upregulated and correlated with sepsis severity.
  • Conversely, genes like XBP1, HERPUD1, LRRK2, and SELENOS were downregulated in septic foals, negatively correlating with sepsis scores and positively with antioxidant enzyme activity.
  • Specific gene correlations were observed, including CREB3L2 with BCL2L1, USP14 with YOD1, and XBP1 with HERPUD1, further elucidating ER stress pathway dynamics in sepsis.

Conclusions:

  • Endoplasmic reticulum (ER) stress is a significant feature of sepsis in neonatal foals, linked to oxidative stress and disease progression.
  • Specific upregulated and downregulated ER stress genes serve as potential biomarkers for sepsis severity and response to antioxidant therapies.
  • Targeting ER stress pathways presents a promising therapeutic avenue and prognostic marker for improving outcomes in septic neonatal foals.