Related Experiment Video
Updated: Sep 10, 2025
10:54
Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
Published on: May 30, 2025
382
Optimizing Yeast Surface-Displayed Unspecific Peroxygenase Production for Sustainable Biocatalysis
Niklas Teetz1, Luc Zuhse1, Dirk Holtmann1
1Process Engineering in Life Sciences 2-Electro Biotechnology, Karlsruhe Institute of Technology, Fritz-Haber-Weg 4, 76131 Karlsruhe, Germany.
Bioengineering (Basel, Switzerland)
|August 28, 2025
Summary
Optimized protocols for yeast surface-displayed unspecific peroxygenases (UPOs) significantly boost biocatalyst activity and stability. These advancements support sustainable industrial applications of UPOs, aligning with global development goals.
Area of Science:
- Biotechnology
- Biocatalysis
- Synthetic Biology
Background:
- Unspecific peroxygenases (UPOs) are key biocatalysts for sustainable oxyfunctionalization processes.
- Efficient immobilization of UPOs on yeast cell surfaces enables versatile applications across scales.
- Optimization of UPO production and handling is crucial for industrial sustainability.
Purpose of the Study:
- To optimize production protocols for yeast surface-displayed UPOs in shaken and stirred systems.
- To establish suitable storage conditions and a sterilization method for UPO biocatalysts.
- To enhance the industrial applicability of UPOs in alignment with UN Sustainable Development Goals.
Main Methods:
- One-factor-at-a-time and design of experiments approaches were employed.
- Protocols for shaken flask and bioreactor cultivations were streamlined and modified.
- Novel parallelized methanol-induced enzyme production in Komagataella phaffii using a BioLector XT® reactor was developed.
Main Results:
- A 60% increase in volumetric activity was achieved in shaken flask cultivations with reduced media.
- Bioreactor cultivations showed at least a doubling of final activity using an alternative medium.
- Recommended sterilization and storage methods maintained or increased enzyme activity over 87 days.
Conclusions:
- Optimized protocols enhance the efficiency and scalability of yeast surface-displayed UPO production.
- The developed methods ensure biocatalyst stability and activity, facilitating industrial adoption.
- This study provides crucial advancements for the sustainable industrial utilization of UPOs.
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