Fluorimetric Determination of Eosin Y in Water Samples and Drinks Using Deep Eutectic Solvent-Based Liquid-Phase...

Area of Science:

• Analytical Chemistry
• Green Chemistry
• Spectroscopy

Background:

• Eosin Y (EY) is a widely used dye requiring sensitive detection methods.
• Traditional analytical methods may lack environmental friendliness or sensitivity.
• Deep Eutectic Solvents (DESs) offer a promising alternative for greener extraction techniques.

Purpose of the Study:

• To develop an environmentally friendly and highly sensitive method for Eosin Y (EY) determination.
• To explore the efficacy of DESs in liquid-liquid microextraction (LPME) for EY analysis.
• To validate the method's performance using fluorescence detection (FLD).

Main Methods:

• Vortex-assisted liquid-liquid microextraction (LPME) utilizing various DESs.
• Comparison of DESs (TBAB with hexanol, octanol, decanol) against conventional solvents.
• Fluorescence detection (FLD) for EY quantification.
• Computational chemistry (DFT, COSMO-RS, Hansen solubility parameters) for theoretical insights.

Main Results:

• The developed LPME-FLD method demonstrated high sensitivity with a limit of detection (LOD) of 0.028 µg·L⁻¹.
• A wide linear calibration range (0.1–130 µg·L⁻¹) and high correlation coefficient (R² = 0.9982) were achieved.
• Excellent precision (RSDs 1.1%–2.7%) and recovery (99.0%–106.2%) were confirmed.
• Successful application to real water samples, validating practical utility.

Conclusions:

• The proposed vortex-assisted LPME-FLD method using DESs is effective and sensitive for EY determination.
• The approach aligns with green chemistry principles, offering an environmentally benign analytical solution.
• The method is suitable for the analysis of EY in complex matrices like real water samples.