High-Sucrose Diet-Induced Reprogramming of Plasma Oxylipin Profiles Reflects Dietary Risk for Gut Barrier...

Area of Science:

• Metabolomics
• Gut Health
• Dietary Impact

Background:

• High-sucrose diet (HSD) impacts colonic oxylipins, contributing to gut barrier dysfunction.
• Systemic oxylipin profile changes due to HSD and their link to gut barrier impairment are not well understood.

Purpose of the Study:

• To investigate the effect of HSD on plasma oxylipin profiles.
• To determine if plasma oxylipins can serve as biomarkers for HSD-induced gut barrier impairment.

Main Methods:

• Analysis of plasma oxylipin profiles in a mouse model fed HSD.
• Correlation analysis of specific oxylipins with gut barrier markers (lipopolysaccharide).
• Comparison of plasma oxylipin profiles in humans with habitual high-sucrose intake versus controls.

Main Results:

• HSD significantly altered plasma oxylipin metabolism, particularly CYP epoxygenase pathway metabolites.
• Plasma 8(9)-epoxyeicosatrienoic acid (8(9)-EET) increased 2.4-fold in mice and 3.7-fold in humans on HSD.
• Elevated 8(9)-EET positively correlated with intestinal impairment and lipopolysaccharide levels in both mice and humans.

Conclusions:

• HSD reprograms plasma oxylipin profiles, with 8(9)-EET elevation as a key finding.
• Increased 8(9)-EET may indicate metabolic stress and serve as an early indicator of dietary sucrose overload.
• Plasma oxylipin profiling, especially 8(9)-EET, offers a potential tool for assessing risks of HSD-induced intestinal injury.