Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

eIF3 Orchestrates a Biphasic Stress Response Linking Translational Control to Mitochondrial Integrity in Skeletal Muscle.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

From Initiation to Elongation: eIF3 as a Dual-Phase Guardian of Mitochondrial Integrity and Protein Homeostasis in Skeletal Muscle.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Suppression of ASNS expression by VHL-mediated ubiquitination hinders the progression of renal cell carcinoma through enhancing JUP expression and inhibiting PI3K-AKT and MAPK pathways.

International journal of biological sciences·2026
Same author

Qingluo Tongbi Formula attenuates Tripterygium wilfordii Hook. f.-induced hepatic metabolic dysfunction and oxidative stress via the SIRT1/HIF-1α pathway.

Journal of ethnopharmacology·2026
Same author

Genetically encoded green-light-responsive photocaged lysine for sequential control of protein function.

Chemical science·2025
Same author

AI-enhanced flexible ECG patch for accurate heart disease diagnosis, optimal wear positioning, and interactive medical consultation.

National science review·2025
Same journal

Retraction: Flucytosine and Amphotericin B Coadministration Induces Dose-Related Renal Injury.

Dose-response : a publication of International Hormesis Society·2026
Same journal

miRNA-Targeted Herbal Compounds Against Gastric Precancerous Evolution in Chronic Atrophic Gastritis.

Dose-response : a publication of International Hormesis Society·2026
Same journal

Plant-Mediated Fabrication of Iron and Zinc Oxide Nanoparticles for Anticancer Efficacy Against HT-29 and HepG2 Cells.

Dose-response : a publication of International Hormesis Society·2026
Same journal

Total <i>Sanghuangporus vaninii</i> Extract Ameliorates Cisplatin-Induced Glioma Cell Death by Regulating Ferroptosis and Inflammation.

Dose-response : a publication of International Hormesis Society·2026
Same journal

Causal Association Between Gut Microbiota, Plasma Metabolites, and Prostate Cancer: Two-Step Mendelian Randomization Study.

Dose-response : a publication of International Hormesis Society·2026
Same journal

DMSO Protects Against Radiation-Induced Ovarian Injury by Preserving Mitochondrial Function and Alleviating DNA Damage.

Dose-response : a publication of International Hormesis Society·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

11.7K

Adenosine Concentration Determination for in Vitro Tumor Microenvironment Simulation.

Chun Chen1, Chuanpeng Yang1, Shuning Hu1

  • 1College of Life Sciences, China Jiliang University, Hangzhou, China.

Dose-Response : a Publication of International Hormesis Society
|August 28, 2025
PubMed
Summary
This summary is machine-generated.

Adenosine (ADO) at 50 μM promotes tumor cell growth and migration, while suppressing natural killer (NK) cell activity and anti-tumor functions. This highlights ADO

Keywords:
NK92 cellsadenosinetumor cellstumor microenvironment

More Related Videos

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.4K
Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography
09:53

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography

Published on: August 16, 2020

7.3K

Related Experiment Videos

Last Updated: Sep 9, 2025

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
16:18

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Published on: November 20, 2011

11.7K
In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.4K
Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography
09:53

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography

Published on: August 16, 2020

7.3K

Area of Science:

  • Immunology
  • Cancer Biology
  • Pharmacology

Background:

  • The tumor microenvironment (TME) plays a crucial role in cancer progression and immune evasion.
  • Adenosine (ADO) is a key molecule within the TME, known to influence cellular functions.
  • Understanding ADO's concentration-dependent effects is vital for developing effective cancer immunotherapies.

Purpose of the Study:

  • To investigate the concentration-dependent effects of adenosine (ADO) on tumor cells and natural killer (NK) cells within the tumor microenvironment (TME).
  • To determine the critical threshold concentration of ADO that modulates tumor immune escape mechanisms.
  • To elucidate the bidirectional regulatory role of ADO in cancer and immunity.

Main Methods:

  • An in vitro simulation system was utilized to assess ADO's impact.
  • Evaluated ADO's effects on A549 (lung carcinoma) and A375 (melanoma) cell proliferation and migration.
  • Assessed ADO's influence on NK92 cell activity, cytokine secretion (IFN-γ), and cytotoxicity against various cancer cell lines (A549, A375, HepG2).

Main Results:

  • A concentration of 50 μM ADO significantly enhanced tumor cell proliferation (60%-80%) and migration (30%-40%).
  • Higher ADO concentrations (>200 μM) demonstrated an inhibitory effect on tumor cells.
  • ADO suppressed NK92 cell activity and IFN-γ secretion in a dose-dependent manner, reducing proliferation by 14.5% and IFN-γ by 24% at 50 μM.
  • ADO impaired NK cell-mediated cytotoxicity against A549, A375, and HepG2 cells by 20.3%, 22.4%, and 31.5%, respectively.

Conclusions:

  • 50 μM ADO serves as a critical threshold concentration for simulating TME effects.
  • ADO exhibits concentration-dependent bidirectional regulation, promoting tumor growth at lower concentrations and potentially inhibiting it at higher ones.
  • ADO significantly impairs NK cell function, contributing to tumor immune escape.