Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.8K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.8K
Tumor Immunotherapy01:27

Tumor Immunotherapy

659
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
659
Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

312
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
312

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying Unmonitored Tone Drift in Patient Education Materials Transcribed to a Lower Reading Level by a Large Language Model.

Neurosurgery·2026
Same author

Machine learning and deep learning-based drug-drug interactions prediction: a systematic review focused on anticancer drugs.

NPJ precision oncology·2026
Same author

Gold Nanoparticles in Central Nervous System Diseases: Recent Progress, Challenges, and Therapeutic Opportunities.

Nano letters·2026
Same author

International multi-center study to quantify the effect of deep venous drainage after surgical resection of Spetzler-Martin Grade II-III brain arteriovenous malformations.

Neurosurgical review·2026
Same author

Cost-Effectiveness of Adjunctive Middle Meningeal Artery Embolization for Chronic Subdural Hematoma: Secondary Analysis of EMBOLISE.

AJNR. American journal of neuroradiology·2026
Same author

Endovascular and Microsurgical Treatment for Middle Cerebral Artery Bifurcation Aneurysms: Experience From 10 High-Volume United States Cerebrovascular Centers.

Neurosurgery·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

791

Developing Aptamer-Targeted mRNA for Immunotherapy.

Alexandra R Paul1,2, Khuloud T Al-Jamal3,4

  • 1Department of Pharmacology and Pharmacy, LKS Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR.

Methods in Molecular Biology (Clifton, N.J.)
|August 28, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel aptamer drug delivery platform for targeted messenger RNA (mRNA) delivery to T-cells. The platform significantly enhances mRNA transfection in immune cells, improving immunotherapy potential.

Keywords:
AptamersFlow cytometryGel electrophoresisImmunotherapyNucleic acidsTransfectionmRNA delivery

More Related Videos

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

3.8K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.7K

Related Experiment Videos

Last Updated: Sep 9, 2025

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

791
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

3.8K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.7K

Area of Science:

  • Biotechnology
  • Immunology
  • Drug Delivery Systems

Background:

  • Messenger RNA (mRNA) therapeutics rely on selective protein expression.
  • Efficient delivery of mRNA to target cells, particularly immune cells, is critical for immunotherapy.
  • Current mRNA delivery methods often lack specificity, limiting therapeutic efficacy.

Purpose of the Study:

  • To develop and validate a novel T-cell-targeting aptamer drug delivery platform for enhanced mRNA delivery to immune cells.
  • To demonstrate the efficiency of aptamer-mediated mRNA transfection in T-cells for immunotherapy applications.
  • To establish protocols for aptamer selection, conjugation, and characterization for mRNA therapeutics.

Main Methods:

  • Aptamer selection and characterization for T-cell targeting.
  • Conjugation of aptamers to OX40 mRNA.
  • Transfection of aptamer-mRNA constructs into murine ex vivo splenocyte T-cells.
  • Quantification of mRNA transfection efficiency in CD4+ and CD8+ T-cells.

Main Results:

  • The T-cell-targeting aptamer significantly enhanced mRNA transfection efficiency in murine T-cells.
  • A fourfold increase in CD4+ cell transfection and a sevenfold increase in CD8+ cell transfection were observed compared to untargeted mRNA.
  • Successful aptamer-mRNA conjugation and characterization were achieved.

Conclusions:

  • The developed aptamer-based platform enables efficient mRNA delivery to T-cells for immunotherapy.
  • This targeted delivery approach enhances immune cell transfection, offering a promising strategy for next-generation mRNA therapeutics.
  • The platform's modularity allows for customization to target other cell types or receptors.