Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

1.6K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
1.6K
Whole Body Regeneration01:33

Whole Body Regeneration

3.6K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
3.6K
Tissue Transplantation01:24

Tissue Transplantation

1.4K
Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An engineered probiotic co-expressing dual melanoma epitopes for therapeutic cancer vaccination.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Engineered Nestin<sup>+</sup> TSPC-Derived Exosomes Promote Tendon Repair Via Metabolic Reprogramming.

Journal of extracellular vesicles·2026
Same author

Rare 19q13.42 duplication encompassing <i>PRKCG</i> associated with neurodevelopmental abnormalities.

Translational pediatrics·2026
Same author

Machine learning-based genome-wide association analysis to construct a clinical decision model for severe neonatal jaundice.

Translational pediatrics·2026
Same author

Spatial and temporal single cell multi-omics in mice reveals macrophage-SFRP4+ stroma interactions promoting endometrial regeneration via TNF-α.

Cell reports·2026
Same author

Platelet-derived vesicles restore fertility in endometrial injury by modulating the endometrial immune niche in mice.

Science advances·2026

Related Experiment Video

Updated: May 6, 2026

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

2.0K

Centimeter-Scale Self-Assembling Tendon Organoids Drive Tissue Regeneration.

Tianshun Fang1,2, Hong Zhang3, Yuanhao Xie1,4,5

  • 1Department of Orthopedic Surgery of Sir Run Run Shaw Hospital, and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|August 29, 2025
PubMed
Summary

Scientists engineered large, transplantable tendon organoids to improve tendon repair. These engineered tissues promote stem cell retention and enhance mechanical properties for better functional recovery after injury.

Keywords:
organoidstem celltendontissue engineering

More Related Videos

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms
08:32

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

1.1K
Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models
03:35

Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models

Published on: June 21, 2024

1.6K

Related Experiment Videos

Last Updated: May 6, 2026

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

2.0K
Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms
08:32

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

1.1K
Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models
03:35

Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models

Published on: June 21, 2024

1.6K

Area of Science:

  • Regenerative Medicine
  • Biomaterials Engineering
  • Stem Cell Biology

Background:

  • Tendon injuries impede tissue regeneration due to limited endogenous stem cells.
  • Restoring tendon structure and function requires effective cell-based therapies.
  • In vitro engineering of large-scale, transplantable stem cell-derived tissues is crucial.

Purpose of the Study:

  • To develop centimeter-scale, transplantable tendon organoids for regenerative medicine applications.
  • To optimize conditions for self-assembly and tenogenic differentiation of stem cells within organoids.
  • To evaluate the efficacy of these organoids in a preclinical tendon defect model.

Main Methods:

  • Optimized chemical signaling and extracellular matrix (ECM) mimicking strategies.
  • Cultured stem cells to form centimeter-scale tendon organoids with high cellular viability.
  • Assessed tenogenic phenotype, ECM production, and lineage-specific differentiation.
  • Implanted organoids in a tendon defect model to evaluate cell retention and repair efficacy.

Main Results:

  • Developed transplantable tendon organoids exceeding 3 cm in human tissue-scale dimensions.
  • Organoids exhibited high cellular viability, proliferation, tenogenic phenotype, and enhanced ECM production.
  • Achieved precise tendon-specific lineage differentiation and retained fetal tendon regenerative capacity.
  • Organoids increased stem cell retention by 7.9 times and promoted denser tendon repair with improved mechanical properties.

Conclusions:

  • Efficient construction of centimeter-scale human tendon organoids with superior regenerative potential was achieved.
  • This strategy offers a promising approach for tendon regeneration and restoration of motor function.
  • Engineered tendon organoids represent a viable therapeutic option for treating tendon injuries.