Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue differences in vascular permeability changes induced by histamine.

P Kilzer, K Chang, J Marvel

    Microvascular Research
    |November 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Small-Vessel Disease: Diabetic Microangiopathy.

    Angiology·2016
    Same author

    Predicting pathogen-specific CD8 T cell immune responses from a modeling approach.

    Journal of theoretical biology·2015
    Same author

    Characterization of Vero cell growth and death in bioreactor with serum-containing and serum-free media.

    Cytotechnology·2008
    Same author

    Loss of p53 or p73 in human papillomavirus type 38 E6 and E7 transgenic mice partially restores the UV-activated cell cycle checkpoints.

    Oncogene·2007
    Same author

    Overexpression of Sp1 transcription factor induces apoptosis.

    Oncogene·2006
    Same author

    A1/Bfl-1 expression is restricted to TCR engagement in T lymphocytes.

    Cell death and differentiation·2003
    Same journal

    Association between retinal microcirculation and cardiac function: A cross-sectional study using OCTA.

    Microvascular research·2026
    Same journal

    Microvascular dysfunction and neurovascular signalling impairment in diabetic silent myocardial ischemia.

    Microvascular research·2026
    Same journal

    Association of circulating biomarkers of glycocalyx injury, sirtuin 1, and NFκB p65 in individuals with type 2 diabetes and concurrent arterial hypertension.

    Microvascular research·2026
    Same journal

    Targeting miR-10 alleviates atherosclerosis by enhancing intestinal ABCA1-mediated cholesterol efflux and repairing intestinal barrier.

    Microvascular research·2026
    Same journal

    The effect of acute high-intensity interval exercise on microvascular function and local skeletal muscle oxygenation.

    Microvascular research·2026
    Same journal

    RNF213 deficiency in human iPSC-derived vascular organoids captures key feature of moyamoya disease vasculopathy.

    Microvascular research·2026
    See all related articles

    This study introduces a new method to measure albumin leakage from blood vessels into tissues after administering vasoactive agents like histamine. Results show histamine significantly increases albumin permeation in the cecum more than in other tissues.

    Area of Science:

    • Physiology
    • Pharmacology
    • Biochemistry

    Background:

    • Vascular permeability is crucial for tissue homeostasis and drug delivery.
    • Vasoactive agents can alter vascular integrity, leading to albumin extravasation.
    • Quantifying tissue-specific changes in albumin permeation is essential for understanding physiological and pathological processes.

    Purpose of the Study:

    • To develop and validate a novel method for assessing albumin permeation across vascular beds in multiple tissues simultaneously.
    • To investigate the tissue-specific effects of histamine on vascular albumin permeability in vivo.
    • To differentiate between filtration and diffusion as mechanisms of albumin extravasation.

    Main Methods:

    • Intravenous injection of radiolabeled red blood cells (51Cr-RBC), albumin (125I-BSA), and EDTA (57Co-EDTA) in animals.

    Related Experiment Videos

  • Intravascular or subcutaneous administration of histamine.
  • Measurement of tissue water content and isotope ratios (125I/51Cr) in blood and tissue samples.
  • Calculation of the tissue-to-blood isotope ratio (TBIR) to quantify albumin distribution volume.

    • Main Results:

    • Histamine significantly increased albumin permeation in the cecum (4-fold at 1.5 mg/kg), exceeding changes in other tissues like skin and muscle.
    • At a higher dose (7.5 mg/kg), histamine increased albumin permeation in a graded manner across various tissues, with the cecum showing the highest increase (5.1x).
    • Increased albumin permeation was primarily mediated by enhanced diffusion, indicated by a greater increase in the 125I/51Cr ratio compared to the 57Co/51Cr ratio.

    Conclusions:

    • The developed method accurately quantifies histamine-induced albumin extravasation in a tissue-specific manner.
    • The cecum exhibits heightened sensitivity to histamine, leading to significantly greater albumin permeation compared to other tissues.
    • Histamine increases vascular permeability mainly through enhanced diffusion of albumin via increased pore size or number.