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Updated: Sep 9, 2025

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
Tailor-Made Dynamic Fluorophores: Precise Structures Controlling the Photophysical Properties
Shuhai Qiu1, Zhiyun Zhang1, Zhaohui Wang1,2
1Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
Organic fluorophores with dynamic conformations in the excited state have played a significant role in applications of organic functional dyes. Among them, dihydrophenazine-based dynamic fluorophores involving a photoinduced structural planarization process in the excited state exhibited large Stokes shifts and conformation-dependent multicolor emissions. With the developments of synthetic strategies, precise modifications on dihydrophenazine-based scaffolds have successfully afforded a variety of precise molecular structures of varying sizes and compositions, which have delicately modulated their photophysical properties. Herein, this Perspective summarizes the precise modulations of dihydrophenazine-based dynamic fluorophores, including the development of the synthetic methodologies, and tailor-made molecular models to reveal the luminescence-structure relationships. Insights about the future molecular design and applications are also presented.
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