A protocol for organoids from the gynecomastia patients

Area of Science:

• Endocrinology
• Cell Biology
• Pathology

Background:

• Gynecomastia is the benign proliferation of male breast glandular tissue with unclear causes.
• Lack of effective in vitro models impedes research into gynecomastia mechanisms and treatments.

Purpose of the Study:

• To establish and characterize organoids derived from human gynecomastia breast tissue.
• To assess the utility of these organoids as a model for studying male breast pathology.

Main Methods:

• Organoids were generated from six male gynecomastia patient tissue samples.
• Histological and molecular analyses (H&E, IHC, IF, qPCR) were performed to evaluate organoid fidelity.

Main Results:

• Organoids successfully recapitulated key histological features and marker expression (CK14, CK18, Ki67, ERα) of the original tissues.
• Protein and transcript levels of markers were largely consistent, with notable post-transcriptional regulation of ERα observed.

Conclusions:

• A reproducible protocol for generating gynecomastia-derived organoids was established.
• These organoids serve as a valuable disease-relevant platform for future research on male breast pathology and hormonal mechanisms.