Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

288
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
288
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.2K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

482
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
482
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photorealistic 3D Holographic Display with Natural Defocus Effect.

Nature communications·2026
Same author

Seeing without touching: weak-disturbance imaging and characterization of ultra-confined optical near fields.

Light, science & applications·2026
Same author

Lensless imaging with a programmable Fresnel zone aperture.

Science advances·2025
Same author

A nanovaccine for immune activation and prophylactic protection of atherosclerosis in mouse models.

Nature communications·2025
Same author

Advances in Axial Resolution Strategies for Super-Resolution Imaging Systems.

Small methods·2025
Same author

Multi-modal transport of intensity diffraction tomography microscopy with an electrically tunable lens [Invited].

Biomedical optics express·2025
Same journal

Bi-layer photonic random meta-composite for cryogenic thermal control by ultra-broadband scattering matched reflectance.

Light, science & applications·2026
Same journal

Interferometric scattering for optical tomoslicing of transparent solids.

Light, science & applications·2026
Same journal

Multi-dimensional spatial-temporal projection ultrafast compressed imaging.

Light, science & applications·2026
Same journal

Expanded field of view light-field extended-reality displays with metalens array.

Light, science & applications·2026
Same journal

Experimental observation of counter-intuitive features of photonic bunching.

Light, science & applications·2026
Same journal

High-speed and high-sensitivity multi-gas detection based on parallel heterodyne LITES sensor.

Light, science & applications·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.4K

Principal component analysis for three-dimensional structured illumination microscopy (PCA-3DSIM).

Jiaming Qian1,2,3, Weiyi Xia1,2,3, Yuxia Huang1,2,3

  • 1Smart Computational Imaging (SCI) Laboratory, Nanjing University of Science and Technology, Nanjing, Jiangsu Province, 210094, China.

Light, Science & Applications
|September 1, 2025
PubMed
Summary
This summary is machine-generated.

PCA-3DSIM enhances 3D structured illumination microscopy (3DSIM) for clearer nanoscale imaging. This new method improves reconstruction accuracy and reduces artifacts, making super-resolution microscopy more robust and practical for biological research.

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.8K
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.8K

Related Experiment Videos

Last Updated: Sep 9, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.4K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.8K
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.8K

Area of Science:

  • Microscopy
  • Super-resolution imaging
  • Biomedical research

Background:

  • Three-dimensional structured illumination microscopy (3DSIM) enables nanoscale visualization of subcellular structures.
  • Reconstruction quality in 3DSIM is often compromised by experimental parameter uncertainties and optical aberrations.

Purpose of the Study:

  • To introduce PCA-3DSIM, a novel framework extending principal component analysis (PCA) to 3D super-resolution microscopy.
  • To enhance the robustness and fidelity of 3DSIM reconstructions.

Main Methods:

  • Developed PCA-3DSIM, a 3D extension of PCA for super-resolution microscopy.
  • Implemented adaptive tiled-block processing to manage spatial nonuniformities in illumination parameters.
  • Segmented volumetric data into localized subsets for parameter estimation and interference rejection.

Main Results:

  • PCA-3DSIM provides high-fidelity, artifact-free 3D super-resolution reconstructions.
  • Demonstrated reliable reconstruction performance and improved robustness across various imaging systems.
  • The tiled reconstruction approach supports efficient processing with limited computational resources.

Conclusions:

  • PCA-3DSIM is a flexible and practical tool for super-resolved volumetric imaging of subcellular structures.
  • Offers broad potential applications in biomedical research by improving 3DSIM robustness.
  • Integrates physical modeling with statistical analysis for a mathematically grounded enhancement to 3DSIM.