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Updated: Sep 9, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

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Published on: March 30, 2018

The miR444f Regulates Root Development via Gibberellin Metabolic Pathway in Rice.

Sheng Huang^1,2, Yuqi Liu¹, Jiyuan Li¹

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.

Plant, Cell & Environment

|September 2, 2025

Summary

This summary is machine-generated.

Loss of microRNA 444f (miR444f) in rice impairs root development by affecting cell division and elongation. This is mediated by gibberellin signaling, highlighting miR444f

Keywords:

MADS‐box gibberellin miR444f rice root development

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Area of Science:

Plant Biology
Genetics
Molecular Biology

Background:

MicroRNAs (miRNAs) are key regulators of plant development.
The MIR444 family's role in rice root development is largely unexplored.
Understanding miRNA functions is crucial for crop improvement.

Purpose of the Study:

To investigate the function of miR444f in rice root development.
To elucidate the molecular mechanisms underlying miR444f-mediated root growth regulation.

Main Methods:

Analysis of miR444f loss-of-function mutants in rice.
Cellular and molecular analyses of root apical meristem.
Gene expression analysis of MADS-box transcription factors and gibberellin metabolic genes.
Exogenous gibberellin treatment experiments.

Main Results:

Loss of miR444f caused significant defects in primary and lateral root development.
miR444f influences root apical meristem activity, including cell division and elongation.
miR444f targets OsMADS27 and OsMADS57, impacting gibberellin metabolism.
Altered expression of GA metabolic genes and changes in GA accumulation were observed.
Exogenous gibberellin application rescued the root growth defects in miR444f mutants.

Conclusions:

miR444f is essential for normal rice root development.
The miR444f-OsMADS-GA signaling pathway is critical for regulating root growth.
Findings provide insights for genetic engineering strategies to enhance crop root architecture and resilience.