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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.1K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

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Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
781

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Related Experiment Video

Updated: Sep 9, 2025

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
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Homogeneous Electrochemical Enzyme-Linked Immunosorbent Assay Strategy Based On pH-Mediated Redox Potential

Jianyang Lu1,2, Yiwei Han2,3, Xiaomeng Yu1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, China.

Chembiochem : a European Journal of Chemical Biology
|September 2, 2025
PubMed
Summary

A new homogeneous electrochemical Enzyme-Linked Immunosorbent Assay (ELISA) offers a cost-effective alternative to traditional methods. This innovation enables sensitive protein detection using simple electrochemical instruments, ideal for point-of-care diagnostics.

Keywords:
pH conditionC‐reactive proteinalkaline phosphataseelectrochemistryenzyme‐linked immunosorbent assay

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biosensors

Background:

  • Enzyme-linked immunosorbent assay (ELISA) is the gold standard for protein detection but requires specialized equipment, limiting its use in resource-limited settings.
  • Electrochemical techniques offer a low-cost, portable alternative but often involve complex electrode modifications and variability.
  • Existing methods face challenges in field applicability due to instrumentation and procedural complexities.

Purpose of the Study:

  • To develop a novel homogeneous electrochemical ELISA platform for simplified and accelerated signal acquisition.
  • To overcome the limitations of conventional electrochemical methods, including complex surface modifications and electrode variability.
  • To demonstrate a versatile, cost-effective approach for point-of-care protein detection.

Main Methods:

  • A homogeneous electrochemical ELISA platform was developed, utilizing pH changes induced by alkaline phosphatase activity.
  • The platform employs methylene blue (MB) as a proton-sensitive electrochemical probe to detect pH variations.
  • Quantitative correlation was established between target protein concentration and MB redox potential shift.

Main Results:

  • Sensitive, reproducible, and quantitative electrochemical detection of C-reactive protein (CRP) was achieved using the developed platform.
  • The novel platform demonstrated simplified and accelerated signal acquisition compared to conventional methods.
  • The system effectively leveraged pH changes and MB redox potential shifts for protein quantification.

Conclusions:

  • The developed homogeneous electrochemical ELISA is a versatile and cost-effective method for protein detection.
  • This approach significantly expands the applicability of ELISA to point-of-care diagnostics and resource-limited environments.
  • The platform offers a promising alternative to traditional optical ELISA systems, enhancing accessibility and usability.